河南农业科学2025，Vol.54Issue(2)：131-137,7.DOI:10.15933/j.cnki.1004-3268.2025.02.015
黄淮肉羊CIDEa和CIDEc基因组织表达与肌内脂肪含量的相关性分析
Correlation Analysis between Tissue Expression of CIDEa and CIDEc Genes and Intramuscular Fat Content in Huanghuai Sheep
摘要
Abstract
The aim of this study was to explore the expression patterns of CIDEa and CIDEc genes in various tissues of Huanghuai sheep,and analyze their correlation with intramuscular fat and fatty acid content.This study selected three male Huanghuai sheep at the ages of 3,9,and 18 months each,collected tissue samples after slaughter and measured their intramuscular fat and fatty acid content.It used RT-qPCR technology to detect CIDEa and CIDEc genes expression level in various tissues and in longissimus dorsi at different ages,and analyzed their correlation with intramuscular fat and fatty acid content using Pearson correlation coefficient.The results showed that both CIDEa and CIDEc had highest expression levels in subcutaneous fat and abdominal fat.The expression level of CIDEa in 4 muscle tissues semitendinosus,longissimus dorsi,triceps brachii and quadriceps femoris increased overall with age.The expression level of CIDEa in the longissimus dorsi was positively correlated with intramuscular fat and C16:0(P<0.05),and negatively correlated with C18:0,C20:2,and C20:4n6(P<0.05).While the expression level of CIDEc gene in longissimus dorsi and triceps brachii decreased with age.The expression level of CIDEc in the longissimus dorsi was positively correlated with C13:0,C18:0,C20:2,C23:0,and C24:0(P<0.05),and negatively correlation with C17:1 and C18:1n9c(P<0.05).In summary,CIDEa and CIDEc genes play important roles in the regulation of intramuscular fat and fatty acids in Huanghuai sheep.关键词
黄淮肉羊/CIDE/组织表达/肌内脂肪Key words
Huanghuai sheep/CIDE/Tissue expression/Intramuscular fat分类
农业科技引用本文复制引用
李君,高福现,蒯子茹,亓玉欣,韩浩园,施会彬,刘昆,权凯..黄淮肉羊CIDEa和CIDEc基因组织表达与肌内脂肪含量的相关性分析[J].河南农业科学,2025,54(2):131-137,7.基金项目
河南省重点研发与推广专项(科技攻关)(232102110007) （科技攻关）
河南省自然科学基金项目(242300420148) （242300420148）
河南省现代农业产业技术体系建设专项(HARS-22-15-S) （HARS-22-15-S）
河南省高校科技创新团队支持计划资助项目(25IRTSTHN027) （25IRTSTHN027）
