检验医学与临床2025，Vol.22Issue(7)：899-904,6.DOI:10.3969/j.issn.1672-9455.2025.07.008
苁蓉总苷对功能性便秘大鼠肠黏膜损伤、ICC、水通道蛋白及胃肠动力学的影响
Effects of total Cistanche glycosides on intestinal mucosal injury,ICC,aquaporin and gastrointestinal dynamics in rats with functional constipation
摘要
Abstract
Objective To study the effects of total Cistanche glycosides on intestinal mucosal injury,Cajal interstitial cells(ICC),aquaporin(AQP)and gastrointestinal dynamics in rats with functional constipation.Methods Among 52 healthy rats,10 rats were randomly selected as the healthy group,and the remaining 42 rats were established as the functional constipation model.After successful model establishment,the rats were randomly divided into model group,total Cistanche glycosides group(model+total Cistanche glycosides 10 mg/kg)and drug control group(model+cisapride 0.1 mL/10 g),with 14 rats in each group.Enzyme linked immunosorbent assay(ELISA)was used to detect gastrointestinal motility related indicators 5-hydroxytryptamine(5-HT),nitric oxide(NO),intestinal substance P(SP)],asoactive intestinal Peptide(VIP).Hematoxylin-Eosin staining was used to detect the pathological changes of colon tissue.qPCR was used to detect the expressions of TLR2mRNA,TLR4mRNA and Claudin-1mRNA in colon tissue.Immunohis-tochemistry was used to detect the ICC marker C-kit in colon tissue.Western blotting was used to detect AQP-related indexes in colonic tissue of rats in each group.Results Compared with the healthy group,the levels of SP,VIP,C-kit AO,AQP4,AQP9 and claudin-1mRNA decreased in the model group,total Cistanche glycosides group and drug control group,and the levels of NO,5-HT,TLR2mRNA,TLR4mRNA,AQP2 and AQP3 increased,the differences were significant(P<0.05).Compared with the model group,the levels of SP,VIP,C-kit AO,AQP4,AQP9 and claudin-1mRNA increased significantly in the total Cistanche glycosides group and the drug control group(P<0.05),and the levels of NO,5-HT,TLR2 mRNA,TLR4 mRNA,AQP2 and AQP3 decreased significantly in the total Cistanche glycosides group and the drug control group(P<0.05).There was no statistical significance in the levels of each index between the total Cistanche glyco-sides group and the drug control group(P>0.05).The colonic mucosa of healthy rats was intact,and the glands were abundant and arranged in an orderly manner.In the model group,the integrity of colonic mucosa was destroyed,the glands were atrophic,and the acini reduced and disorganized.The pathological changes of total Cistanche glycosides group and drug control group were improved compared with model group.Conclu-sion Total Cistanche glycosides may play a role in the treatment of functional constipation by regulating in-testinal neurotransmitters,aquaporin-associated proteins,ICC and improving intestinal mucosal injury.关键词
苁蓉总苷/功能性便秘大鼠/肠黏膜损伤/Cajal间质细胞/水通道蛋白/胃肠动力学Key words
total Cistanche glycosides/functional constipation/intestinal mucosal injury/Cajal inter-stitial cell/aquaporin/gastrointestinal dynamics分类
医药卫生引用本文复制引用
于贺美,韩晴,黄贺明..苁蓉总苷对功能性便秘大鼠肠黏膜损伤、ICC、水通道蛋白及胃肠动力学的影响[J].检验医学与临床,2025,22(7):899-904,6.基金项目
河北省中医药管理局科研计划项目(2022278). （2022278）
