青岛大学学报（自然科学版）2025，Vol.38Issue(1)：1-7,23,8.DOI:10.3969/j.issn.1006-1037.2025.01.01
circRNA-7364调控胃癌自噬的作用机制研究
Study on the Mechanism of CircRNA-7364 Regulating Autophagy in Gastric Cancer
摘要
Abstract
To investigate the functional mechanism of circular RNA hsa_circ_0007364(circ-7364)which was identified in the preliminary research results in regulating autoph-agy and the malignant phenotype of gastric cancer.Circ-7364 associated with autophagy was identified using a doxorubicin-induced autophagy model in human gastric cancer cells(SGC-7901)through real-time PCR,circRNA ring formation identification,and sequen-cing.Characterization involved RNase R tolerance assays,real-time quantitative PCR,and immunofluorescence.Western blotting and immunofluorescence were employed to assess the impact of circ-7364 knockdown on autophagy levels in SGC-7901 cells.The effects on malignant phenotypes were evaluated using CCK-8 proliferation assays,colony formation assays,and Transwell assays.Results showed that autophagy marker proteins were sig-nificantly upregulated in clinical gastric cancer tissues and cell lines compared to normal tissues and cells.Circ-7364 is primarily localized in the cytoplasm and exhibits high stabili-ty.In the SGC-7901 autophagy model,circ-7364 expression was significantly downregu-lated.Knockdown of circ-7364 led to increased expression of autophagy marker protein LC3 and autophagy receptor protein P62,while inhibiting cell proliferation,colony forma-tion,cell migration and invasion.关键词
环状RNA/胃癌/自噬/自噬流受阻/癌症恶性表型Key words
circular RNA/gastric cancer/autophagy/disruption of autophagic flux/malig-nant cancer phenotypes分类
生物科学引用本文复制引用
王媛,单婵..circRNA-7364调控胃癌自噬的作用机制研究[J].青岛大学学报（自然科学版）,2025,38(1):1-7,23,8.基金项目
山东省自然科学基金面上项目(批准号:ZR2022MH032)资助. （批准号:ZR2022MH032）
