广东石油化工学院学报2025，Vol.35Issue(1)：5-11,7.
MIL-53(Al)和cMIL-53(Al)脂解酶微反应器构建及催化扁桃酸应用
Construction of MIL-53(Al)and cMIL-53(Al)Lipolysis Microreactor and Application of Mandelic Acid Catalysis
摘要
Abstract
In recent years,there has been significant interest in the enantioselective hydrolysis of mandelic acid by lipases.However,long reaction times and low reusability have hindered its practical application.Therefore,this study proposes a novel approach utilizing metal-organic framework(MOF)and their carbonized counterparts(cMOF)for immobilizing lipase(BCL)and investigates their catalytic performance and enzyme immobilization mechanism.The results demonstrate that cMIL-53(Al)immobilized BCL after high-temperature carbonization exhibits superior catalytic efficiency,maintaining a 50%conversion rate of(R)-mandelic acid in nine consecutive cycles,with an enantiomeric excess of product(eep%)exceeding 99%and an enantioselectivity factor(E)greater than 200.Further investigation reveals that the surface of carbonized cMIL-53(Al)is rich in COOH functional groups and possesses larger microporous structures,facilitating the entry of BCL into its structure and enabling efficient and repetitive catalysis of mandelic acid through hydrogen bonding with COOH.The enzyme immobilization method proposed in this study offers simplicity in preparation,reusability,and ease of product purification and separation,thus providing a novel strategy for the development of functional materials.关键词
金属有机骨架材料/MIL-53(Al)/碳化/酶固定化/微反应器Key words
metal-organic framework/MIL-53(Al)/carbonizing/immobilized enzyme/microreactor引用本文复制引用
李远华,凌思棋,朱永娟,唐溢杰,谢世桓..MIL-53(Al)和cMIL-53(Al)脂解酶微反应器构建及催化扁桃酸应用[J].广东石油化工学院学报,2025,35(1):5-11,7.基金项目
广东省自然科学基金项目(1515010074) （1515010074）
惠州学院博士科研启动项目(2020JB030) （2020JB030）
广东省大学生创新训练项目(S202310577058) （S202310577058）
