四川大学学报（自然科学版）2025，Vol.62Issue(2)：271-278,8.DOI:10.19907/j.0490-6756.250025
猕猴桃性别分子标记鉴定与开发
Identification and development of molecular markers for sex in kiwifruit
摘要
Abstract
Kiwifruit is a perennial vine,and its dioecious nature makes sex identification important in its breeding process.This study collected and evaluated three sets of developed molecular markers for sex identi-fication in kiwifruit:SmY1,S1032 and FrBy.The results showed that the accuracy of SmY1 and FrBy was 25%for male plants and 100%for female plants,while S1032 failed to amplify specific bands in both male and female samples,and did not show obvious sex specificity.In this study,a pair of new primers,Fr-1,was designed and screened to identify male and female plants of 89 kiwifruit samples with known sexes with 100%accuracy.Furthermore,when applied to seedling sex identification,Fr-1 amplified stable bands,dem-onstrating its practical application value.The Fr-1 marker provides accurate and efficient sex identification in kiwifruit,and can be widely used in breeding practice.关键词
猕猴桃/雌雄株/性别鉴定/分子标记Key words
Kiwifruit/Dioecism/Sex determination/Molecular markers分类
农业科技引用本文复制引用
温淳,魏嘉鸿,赵伊然,曾涛,李明章,张卉,刘志斌,谢玥..猕猴桃性别分子标记鉴定与开发[J].四川大学学报（自然科学版）,2025,62(2):271-278,8.基金项目
四川省科技计划项目(2021YFYZ0010) （2021YFYZ0010）
