经济动物学报2025，Vol.29Issue(2)：152-158,7.DOI:10.13326/j.jea.2025.2019
猫杯状病毒CC19-01株的分离鉴定及遗传进化分析
Isolation,Identification and Evolution Analysis of CC19-01 Strain of Feline Calicivirus
摘要
Abstract
In order to understand the epidemiological characteristics and genetic evolution of feline calicivirus(FCV),samples of conjunctival and nasal swabs from cats suspected upper respiratory tract disease in a pet hospital in Changchun were collected for virus isolation.The viruses were identified using dual-Nano PCR,colloidal gold test strip,and indirect immunofluorescence method.The isolated strains were then subjected to whole-genome amplification and genetic evolution analysis.Subsequently,artificial infection experiments were conducted.The results indicated that the F81 cells in the test group exhibited the cytopathic effect(CPE)such as cell rounding,grape-like clusters,and detachment.In the immunofluorescence assay,the F81 cells in the test group displayed specific green fluorescence,indicating the successful isolation of a feline calicivirus strain,which was hereby designated as the CC19-01 strain.The homology of the CC19-01 strain with known FCVs worldwide ranged from 76.4%to 83.6%.The genome of this strain is highly variable.It had a close phylogenetic relationship with the classic strain MZ712017.1,MZ712021.1,MZ712018.1 isolated in Nanning and was on a parallel evolutionary branch with the vaccine strains JX519210.1(America)and KM111171.1(America).The results of the artificial infection experiments showed that cats developed symptoms 3 to 7 days after infection with this strain,with increased body temperature,increased ocular discharge,loss of eyelashes around the eyes,decreased appetite,and lethargy.The clinical symptoms of infected cats began to recover after 7 days.These results indicated that the genome of the CC19-01 strain of feline calicivirus was highly variable and not lethal to cats.The experiment not only enriched the basic data of domestic FCV but also provided significant molecular information for the development of FCV vaccine and the prevention and control of the disease.关键词
猫上呼吸道感染/猫杯状病毒/分离鉴定/同源性比对/遗传进化分析Key words
feline upper respiratory tract disease/feline calicivirus/isolation and identification/homology comparison/phylogenetic evolution analysis分类
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闫曼平,冯二凯,单晓枫,程悦宁,吴顺,罗国良,王振军,王春霞..猫杯状病毒CC19-01株的分离鉴定及遗传进化分析[J].经济动物学报,2025,29(2):152-158,7.基金项目
国家重点研发计划项目(2023YFD1800702),吉林省科技发展计划项目(20210202099NC) （2023YFD1800702）
