科技创新与应用2025，Vol.15Issue(9)：60-63,68,5.DOI:10.19981/j.CN23-1581/G3.2025.09.014
重组毕赤酵母表达杂合抗菌肽HsSP-TtT1
摘要
Abstract
To construct a recombinant Pichia pastoris pPICZαA-HsSP TtT1/X33 and express the hybrid antibacterial peptide HsSP TtT1.Based on bioinformatics analysis and the codon preference of Pichia pastoris,the nucleic acid sequence of the hybrid antimicrobial peptide HsSP TtT1 was designed,the recombinant plasmid pPICZ α A-HsSP TtT1 was constructed,and Pichia pastoris X33 was transformed.The positive transformants were screened using the bleomycin resistant YPD plate.After methanol induction,they were verified by SDS-PAGE gel electrophoresis.The recombinant plasmid pPICZαA-HsSP-TtT1 were successfully constructed,and a recombinant strain of Pichia pastoris X33/pPICZαA-HsSP-TtT1 which could effectively express the target protein was successfully screened.This study provides a material basis for the efficient preparation of the hybrid antimicrobial peptide HsSP-TtT1.关键词
杂合抗菌肽/毕赤酵母/重组表达/HsSP/TtT1Key words
hybrid antibacterial peptide/pichia pastoris/recombinant expression/HsSP/TtT1分类
生物科学引用本文复制引用
上官雪茹,蔡依婕,李婉祺,张海乾,陈伟峰,曾臻,谭强来..重组毕赤酵母表达杂合抗菌肽HsSP-TtT1[J].科技创新与应用,2025,15(9):60-63,68,5.基金项目
福建省自然科学基金青创项目(2022J05322) （2022J05322）
福建省中青年教师教育科研项目(JAT210492) （JAT210492）
厦门医学院大学生创新创业训练计划项目(202212631005,202212631006,202412631031) （202212631005,202212631006,202412631031）
