热带亚热带植物学报2025，Vol.33Issue(2)：177-186,10.DOI:10.11926/jtsb.4880
基于非靶向代谢组学的茶树新品系'春绿'代谢物分析
Change of Metabolites of New Tea Strain'Chunlv'Based on Untargeted Metabolomics
摘要
Abstract
In order to investigate the composition of metabolites in the leaves of a new tea strain of'Chunlv',the metabolites in the leaves of'Chunlv'and mother tree('Fuyun 6')were analyzed by using LC-MS and GC-MS.The results showed that 50 metabolites with significant differences(VIP>1.0)were identified by GC-MS,mainly including sugars and amino acids,among which 9 were up-regulated and 41 were down-regulated.The chlorogenic acid content had the greatest difference.There were 297 metabolites with significant differences(VIP>1.0)identified by LC-MS,176 up-regulated and 121 down-regulated,mainly concentrated in polyphenols,alkyds and sugars,among which caffeine had the greatest difference.Pathway enrichment analysis showed that the differential metabolites were mainly enriched in the biosynthesis pathway of flavonoids and flavonols,the biosynthesis pathway of arginine,the biosynthesis pathway of unsaturated fatty acids,and the mutual conversion pathway of pentose and glucuronic acid.Variable contribution analysis of OPLS-DA showed that most of the potential biomarkers were related to energy metabolism and quality,linoleic acid,oleic acid,phytol,hydroxypiperidolic acid,ribosol,xylose were related to energy metabolism,chlorogenic acid,tryptophan,caffeine,theophylline and so on were closely related to quality.The analysis of metabolites and metabolic pathways provided a theoretical basis for the quality formation and variety breeding of'Chunlv'.关键词
茶树新品系/代谢物/非靶向代谢组学Key words
New tea strain/Metabolite/Untargeted metabolomics引用本文复制引用
钟秋生,单睿阳,林郑和,黄婷,李鑫磊,陈常颂..基于非靶向代谢组学的茶树新品系'春绿'代谢物分析[J].热带亚热带植物学报,2025,33(2):177-186,10.基金项目
福建省属公益类专项(2021R1029006) （2021R1029006）
福建省自然科学基金项目(2020J011365) （2020J011365）
国家茶叶产业技术体系项目(CARS-19) （CARS-19）
"5511"协同创新工程(XTCXGC2021004)资助This work was supported by the Special Project for Public Welfare Research Institutes in Fujian(Grant No.2021R1029006),the Project for Natural Science in Fujian(Grant No.2020J011365),the National Tea Industry Technology System Project(Grant No.CARS-19),and the"5511"Collaborative Innovation Project(Grant No.XTCXGC2021004). （XTCXGC2021004）
