陕西中医2025，Vol.46Issue(4)：440-444,5.DOI:10.3969/j.issn.1000-7369.2025.04.002
扶金化积丸含药血清对肺癌A549细胞增殖及凋亡的影响
Effect of Fujin Huaji pill containing serum on proliferation and apoptosis of lung cancer A549 cells
摘要
Abstract
Objective:To investigate the effects of Fujin Huaji pill containing serum on the proliferation and ap-optosis of lung cancer A549 cells and the possible molecular mechanism.Methods:A total of 40 SD rats were random-ly divided into blank group and Fujin Huaji pill group.The blank group was given equal volume of normal saline by gavage,and the drug administration group was given gavage according to the drug dose of 1.749 g/kg.Serum contai-ning different concentrations of SD was extracted and allocated to high,medium and low dose groups(150,75 and 37.5 mg/ml of SD serum).Cell line A549 was resuscitated,and the cells of log phase were inoculated into 6-well cul-ture plates at a density of 5×104/ml,and then cultured until the cells stuck to the wall.The next day,each experi-mental group was added 500μl/well of drug-containing serum in the high-dose,medium-dose and low-dose of Fujin Huaji pill,respectively.After 24 hours of intervention,the morphological changes of cells were observed under micro-scope and photographed for analysis.MTT assay was used to detect the proliferation inhibition effect of drug-contai-ning serum on A549 cells.The effects of drugs on cell cycle and apoptosis were detected by flow cytometry.The ex-pression levels of apoptotic proteins Bax and Bcl-2 were detected by Western blot.Results:Compared with the blank group,Fujin Huaji pill serum dose group showed nuclear pyretic,round cell number and floating,cytoplasm clear and granules in the cytoplasm,and cell adhesion was not firm,among which the effect of Fujin Huaji pill serum high and medium dose group was obvious.OD values in high,medium and low dose groups of Fujin Huaji pill were significant-ly decreased compared with blank control group(P<0.01 or P<0.05).The percentage of A549 cells in G0/G1stage was significantly increased in high and medium dose groups,and the percentage of A549 cells in S and G2/M stage was significantly decreased in high dose groups(P<0.01 or P<0.05).The apoptosis rate of A549 cells in high,me-dium and low dose groups of Fujin Huaji pill was significantly increased(P<0.01 or P<0.05).The expression of Bax and the proportion of Bax/Bcl-2 in serum high,medium and low dose groups of Fujin Huaji pill were up-regula-ted(P<0.01),and the expression of Bcl-2 in serum high and medium dose groups of Fujin Huaji pill was down-reg-ulated compared with blank control group(P<0.01 or P<0.05).Conclusion:The serum containing Fujin Huaji pill can inhibit the proliferation of A549 cells and promote the apoptosis of A549 cells,which may be related to the de-crease of Bcl-2 gene level and the increase of Bax gene level.关键词
肺癌/扶金化积丸/细胞增殖/凋亡/细胞周期/含药血清Key words
Lung cancer/Fujin Huaji pills/Cell proliferation/Apoptosis/Cell cycle/Drug serum分类
临床医学引用本文复制引用
付芸芸,雷旭东,夏小军,秦大凯,郭炳涛,付菲菲,任德祥,赵峻,李炜,李林洲..扶金化积丸含药血清对肺癌A549细胞增殖及凋亡的影响[J].陕西中医,2025,46(4):440-444,5.基金项目
甘肃省自然科学基金资助项目(23JRRA1252) （23JRRA1252）
甘肃省科技厅重点研发计划项目(24YFFA019) （24YFFA019）
甘肃省中医药人才培养重点学科建设项目[甘财社(2022)48号] （2022）
甘肃省名中医传承工作室建设项目[国中医药规财函(2021)242] （2021）
