陕西中医2025，Vol.46Issue(4)：450-455,6.DOI:10.3969/j.issn.1000-7369.2025.04.004
八桂止痛散对带状疱疹后遗神经痛大鼠脊髓背角组织JNK/p38 MAPK/NF-κB p65通路的影响
Effect of Bagui Zhitong powder on the JNK/p38 MAPK/NF-κB p65 pathway in spinal dorsal horn tissue of rats with postherpetic neuralgia
摘要
Abstract
Objective:To investigate the effect of Bagui Zhitong powder on the JNK/MAPK/NF-κB pathway in the spinal dorsal horn tissue of rats with postherpetic neuralgia(PHN).Methods:SD rats were applied to prepare a PHN model by intraperitoneal injection of resin toxin(RTX).They were randomly separated into a control group,a model group,a low-dose Bagui Zhitong powder group,a medium dose Bagui Zhitong powder group,and a high-dose Bagui Zhitong powder group,with 10 rats in each group,another 10 SD rats as the control group,after intervention treatment with different doses of Bagui Zhitong powder,long-term spontaneous pain and hyperalgesia symptoms in rats were detected.The positive expression of GFAP activation markers and JNK/p38 MAPK/NF-κB p65 pathway related proteins in the spinal dorsal horn tissue of rats were detected by immunofluorescence staining.The levels of inflammatory factors in the spinal dorsal horn tissue and serum of rats were detected by ELISA.The expression of JNK/p38 MAPK/NF-κB p65 pathway related proteins in the spinal dorsal horn tissues of rats were detected by im-munoblotting.Results:Compared with the control group,the number of spontaneous foot contraction reflexes,relative positive expression of GFAP,p-JNK,p-p38 MAPK,and p-NF-κB p65 in the spinal dorsal horn tissue,the levels of in-terleukin-6(IL-6)and cyclooxygenase-2(COX-2)in serum and spinal dorsal horn tissue,and p-JNK/JNK,p-p38 MAPK/p38 MAPK,p-NF-κB p65/NF-κB p65 in the spinal dorsal horn tissue of rats in the model group were obvi-ously increased(P<0.05),the mechanical pain threshold and thermal pain threshold were obviously reduced(P<0.05).Low,medium,and high-doses of Bagui Zhitong powder can all alleviate the changes in the above pathological indicators in PHN rats(P<0.05),and were dose-dependent(P<0.05).Conclusion:Bagui Zhitong powder can inhibit the activation of astrocytes in the spinal dorsal horn tissue of PHN rats,thereby reducing the level of inflammatory response,alleviating spontaneous pain and hyperalgesia symptoms in rats which may be achieved by blocking the acti-vation of the JNK/p38 MAPK/NF-κB p65 signaling pathway.关键词
带状疱疹后遗神经痛/八桂止痛散/脊髓背角组织/星形胶质细胞/炎症/环氧化酶-2Key words
Postherpetic neuralgia/Bagui Zhitong powder/Spinal dorsal horn tissue/Astrocytes/Inflamma-tion/Cyclooxygenase-2分类
临床医学引用本文复制引用
廖承成,尹瑞文,张洪卫,张爽,李霞,彭国瑶,聂欣怡..八桂止痛散对带状疱疹后遗神经痛大鼠脊髓背角组织JNK/p38 MAPK/NF-κB p65通路的影响[J].陕西中医,2025,46(4):450-455,6.基金项目
云南省科技厅科技计划项目(202001AZ070001-053) （202001AZ070001-053）
云南省教育厅科学研究基金资助项目(2023Y0488) （2023Y0488）
云南省冯素英专家工作站建设项目(202105AF150035) （202105AF150035）
云南省中医医院卓越人才项目[云中附党字(2021)5号] （2021）
