南京林业大学学报（自然科学版）2025，Vol.49Issue(2)：12-22,11.DOI:10.12302/j.issn.1000-2006.202403036
文冠果BZR1基因家族鉴定及功能分析
The identification and functional analysis of BZR1 genes in yellowhorn
摘要
Abstract
[Objective]This study aimed to systematically characterize the BZR1 transcription factor family in yellowhorn(Xanthoceras sorbifolium)and elucidate its functional roles in abiotic stress response,thereby providing insights for breeding stress-resistant cultivars.[Method]Genome-wide identification of XsBZR 1 genes was performed using bioinformatics tools.Subcellular localization of XsBZR1 proteins was validated via 35S::XsBZR1-eYFP fusion constructs.Expression profiles under abiotic stresses(low temperature,salt,drought)and abscisic acid(ABA)treatment were analyzed by qRT-PCR.Functional validation was conducted by overexpressing XsBZR1-9in Arabidopsis thaliana and assessing salt tolerance phenotypes.[Result]Nine XsBZR1 genes(XsBZR 1-1 to XsBZR 1-9)were identified and distributed unevenly across six chromosomes.Phylogenetic and synteny analyses revealed close evolutionary relationships with dicotyledonous BZR1 orthologs.Promoter regions harbored abundant cis-regulatory elements associated with light responsiveness(48.9％),hormone signaling(35.0％)and stress adaptation(24.6％).The nuclear localization of all XsBZR1 proteins was experimentally confirmed.Differential expression patterns were observed under stress conditions:low temperature(4℃)rapidly induced eight XsBZR1 genes(1.3-to 6.0-fold upregulation at 3 h;P＜0.05),except XsBZR 1-7.Salt stress(150 mmol/L NaCl)suppressed XsBZR 1-3/4/5/7 but strongly upregulated XsBZR 1-1(26.1-fold)and XsBZR 1-9(19.6-fold)at 9 h(P＜0.001).Drought stress(mass fraction 25％PEG6000)elevated XsBZR 1-3/7/8/9expression(＞1.9-fold at 9 h),while others remained stable.ABA treatment(100 μmol/L)universally induced XsBZR1 genes,with XsBZR 1-8showing a 35.4-fold increase(P＜0.001).Transgenic Arabidopsis overexpressing XsBZR 1-9exhibited enhanced salt tolerance,with taproot lengths twice that of the wild-type under 100 mmol/L NaCl(P＜0.01).[Conclusion]The XsBZR1 gene family plays pivotal roles in yellowhorn's abiotic stress response,with XsBZR 1-9 demonstrating significant potential for improving salt tolerance.These findings advance the molecular understanding of stress adaptation mechanisms in woody plants and provide targets for precision breeding.关键词
文冠果/BZR1转录因子/非生物胁迫/基因过表达/盐胁迫响应/XsBZR 1-9Key words
yellowhorn(Xanthoceras sorbifolium)/BZR1 transcription factor/abiotic stress tolerance/gene overexpression/salt stress response/XsBZR1-9分类
林学引用本文复制引用
许慧慧,班卓,王晨雪,毕泉鑫,刘肖娟,王利兵..文冠果BZR1基因家族鉴定及功能分析[J].南京林业大学学报（自然科学版）,2025,49(2):12-22,11.基金项目
国家自然科学基金项目(32271838). （32271838）
