南京林业大学学报（自然科学版）2025，Vol.49Issue(2)：23-30,8.DOI:10.12302/j.issn.1000-2006.202309001
文冠果XsWRI1基因克隆、转录活性及组织特异性表达分析
Cloning,transcriptional activation,and tissue expression analysis of XsWRI1 from Xanthoceras sorbifolium
摘要
Abstract
[Objective]This study aimed to characterize the sequence identity,transcriptional activation potential,and functional domains of the WRINKLED 1(WRI1)transcription factor in Xanthoceras sorbifolium(yellowhorn),providing insights into its regulatory role in seed oil biosynthesis.[Method]The full-length cDNA of XsWRI1 was cloned from endosperm tissues of mature yellowhorn using rapid amplification of cDNA ends(RACE).Bioinformatics tools were employed to analyze protein sequence properties.A recombinant pGBKT7-XsWRI1 vector was constructed and transformed into Y2HGold yeast cells to assess transcriptional activation activity.Tissue-specific expression patterns were quantified via quantitative real-time PCR(qRT-PCR)across the root,stem,leaf,petal,stamen and developing embryo tissues.[Result]The XsWRI1 gene(GenBank accession:OR500287)spans 1 688 bp,encoding a hydrophilic and unstable protein of 414 amino acids.Yeast activity testing confirmed strong transcriptional activation activity of XsWRI1.Tissue-specific expression analysis revealed predominant XsWRI1 expression in developing embryos,with negligible levels in vegetative organs(root,stem,leaf)and reproductive structures(petal,stamen).Structural prediction identified two conserved AP2/EREBP DNA-binding domains(residues 76-148 and 177-238)and a nuclear localization signal.[Conclusion]This study elucidates the molecular characteristics and tissue-specific regulatory role of XsWRI1 in yellowhorn,highlighting its potential function in lipid biosynthesis pathways.These findings establish a foundation for targeted genetic manipulation to enhance seed oil accumulation in woody oil crops.关键词
文冠果/XsWRI1/基因克隆/转录激活/组织特异性表达/种子油生物合成Key words
Xanthoceras sorbifolium/XsWRI1/gene cloning/transcriptional activity/tissue-specific expression/seed oil biosynthesis分类
林学引用本文复制引用
张薇,李麟坤,梁重钧,王利兵..文冠果XsWRI1基因克隆、转录活性及组织特异性表达分析[J].南京林业大学学报（自然科学版）,2025,49(2):23-30,8.基金项目
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