实用中医内科杂志2025，Vol.39Issue(3)：33-36,后插3-后插7,9.DOI:10.13729/j.issn.1671-7813.Z20230138
基于UPLC-Q-TOF-MS技术探讨溃疡性结肠炎模型大鼠结肠组织代谢通路及代谢物质
Exploring the Metabolic Pathways and Metabolites in Colon Tissue of Ulcerative Colitis Model Rats Based on UPLC-Q-TOF-MS Technology
摘要
Abstract
Objective To investigate the differences in colonic tissue metabolites in a rat model of ulcerative colitis(UC)and explore the associated metabolic pathways and metabolites underlying UC pathogenesis.Methods Metabolomic analysis was per-formed using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).Identified metabolites were annotated and analyzed for KEGG metabolic pathways via MetaboAnalyst 5.0 based on the KEGG Pathway database.Results Eight metabolic pathways closely linked to UC were identified:the pentose phosphate pathway,galactose metabolism,alanine/aspartate/glutamate metabolism,arginine/proline metabolism,beta-alanine metabolism,arachi-donic acid metabolism,fatty acid metabolism,and fatty acid biosynthesis.Ten associated metabolites were determined:malonic acid,2-deoxyribose 1-phosphate,(±)5,6-dihydroxy-8Z,11Z,14Z-eicosatrienoic acid,L-asparagine,4-guanidinobu-tyric acid,5-aminovaleric acid,guanidinoacetic acid,N-acetyl-D-galactosamine,and delta-12-prostaglandin J2.Conclu-sion In Ulcerative Colitis development,long-term intestinal oxidative stress impairs the mucosal barrier,disrupting the metab-olism of energy substances like amino acids and carbohydrates.关键词
溃疡性结肠炎/超高效液相色谱-四极杆-飞行时间串联质谱法/代谢组学/疾病机制Key words
UC(ulcerative colitis,UC)/UPLC-Q-TOF-MS/metabolomics/disease mechanism分类
医药卫生引用本文复制引用
王佳林,马秀兰,朱西杰,李卫强..基于UPLC-Q-TOF-MS技术探讨溃疡性结肠炎模型大鼠结肠组织代谢通路及代谢物质[J].实用中医内科杂志,2025,39(3):33-36,后插3-后插7,9.基金项目
国家自然科学基金项目(81760863 （）
81960859) （）
宁夏回族自治区自然科学基金项目(2024A2068) （2024A2068）
宁夏回族自治区高等学校教育厅建设项目(NGY2017111) （NGY2017111）
