土壤学报2025，Vol.62Issue(1)：114-126,13.DOI:10.11766/trxb202309100369
棉隆熏蒸和强还原处理对农田土壤抗生素抗性基因的影响研究
Effects of Dazomet Fumigation and Reductive Soil Disinfestation on Antibiotic Resistance Genes in Farmland Soil
摘要
Abstract
[Objective]The prevalence and dissemination of antibiotics resistance genes(ARGs)in farmland soils have become a major threat to food security and human health.However,there is still no effective method to remediate ARGs-contaminated farmland soil.Chemical fumigation and reductive soil disinfestations(RSD)are widely used to kill soil-borne pathogens in agricultural production,but it is still unknown whether they are capable of reducing the abundance of ARGs in ARGs-enriched soil.[Method]In this study,an ARGs-enriched farmland soil due to long-term application of chicken manure was selected,and soil incubation experiment with seven treatments:CK(control without soil treatment),FCK(maximum water holding capacity treatment),DZ(chemical fumigation with 0.02％dazomet),and RSD with 1％ethanol(ET,TOC:521.7 g·kg-1),alfalfa(AL,TOC:454.9 g·kg-1,C/N:21.2),molasses(MO,TOC:270.1 g·kg-1,C/N:12.6)and the mixture of alfalfa and molasses(AM,m/m=1∶1),were conducted to investigate the shifts in absolute abundance and relative abundance of ARGs and mobile genetic elements(MGEs)via real-time PCR.The effects of different treatments on soil ARGs and MGEs were evaluated by reduction rate.[Result]Results showed that RSD treatment could decrease the relative abundance of aadA21,msrE,tetG,tetM,and ErmF genes,with the reduction of the aadA21 gene in relative abundance being 50.5％-58.3％in AL-,MO-,and AM-treated soils,while the relative abundances of msrE,tetG,and tetM genes were significantly lowered by ET treatment,with the reduction rate being as high as 80.9％,78.3％,and 66.9％,respectively.Meanwhile,RSD treatment could significantly decrease the relative abundance of MGEs(IS6100 and IS26 gene),with the reduction rate being 72.5％-76.2％and 38.4％-56.2％,respectively.In addition,the relative abundances of ARGs and MGEs were slightly increased in DZ treatment,with the increasing rate of ARGs and IS26 gene being 21.9％and 60％,respectively.[Conclusion]Collectively,RSD treatment can decrease soil ARGs contamination by reducing the relative abundance of ARGs and MGEs,limiting the horizontal transfer ability of ARGs,and the reduction effect is related to the type of organic materials used.Moreover,RSD treatment is more effective in reducing the relative abundance of ARGs and MGEs than dazomet fumigation and has the potential for rapid remediation of ARGs-contaminated soil.关键词
抗生素抗性基因/可移动遗传元件/棉隆熏蒸/强还原处理/相对丰度Key words
Antibiotic resistance genes/Mobile genetic elements/Dazomet fumigation/Reductive soil disinfestation/Relative abundance分类
资源环境引用本文复制引用
于文豪,李舒,林于蓝,张晶清,徐晨伟,刘亮亮,张金波,蔡祖聪,赵军..棉隆熏蒸和强还原处理对农田土壤抗生素抗性基因的影响研究[J].土壤学报,2025,62(1):114-126,13.基金项目
国家自然科学基金项目(42090065,42077031)、江苏省农业农村污染防治技术与装备工程研究中心开放资金(GCZXZD2301)和江苏省研究生科研与实践创新计划项目(KYCX23_1720,KYCX22_1568)资助 Supported by the National Natural Science Foundation of China(Nos.42090065,42077031),the Opening Project of Jiangsu Province Engineering Research Center of Agricultural and Rural Pollution Prevention Technology and Equipment(No.GCZXZD2301),and the Postgraduate Research & Practice Innovation Program of Jiangsu Province(Nos.KYCX23_1720,KYCX22_1568) （42090065,42077031）
