汕头大学医学院学报2025，Vol.38Issue(1)：1-5,5.DOI:10.13401/j.cnki.jsumc.2025.01.001
依达拉奉对3-异丁基-1-甲基黄嘌呤诱导的B16细胞黑色素生成的影响
Effect of edaravone on 3-isobutyl-1-methylxanthine-induced melanogenesis in B16 cells
摘要
Abstract
Objective:To investigate the effect of edaravone on melanogenesis in B16 cells induced by 3-isobutyl-1-methylxanthine(IBMX).Methods:B16 cells were treated with 75 μmol/L IBMX and then exposed to edaravone at concentrations of 0,25,and 100 μmol/L,respectively.After co-culture for 72 hours,the expression of the melanin marker HMB-45 was observed by immunohistochemistry,melanin content was measured by NaOH lysis method,tyrosinase protein expression was analyzed by Western blot,and tyrosinase activity was detected by the dopa oxidation method.Results:Under IBMX induction,the activity of tyrosinase increased,the expression of HMB-45 was upregulated,and melanin content increased in B16 cells(all P<0.05).After the addition of edaravone,tyrosinase activity decreased,the expression of HMB-45 was downregulated,and melanin content reduced,and showed a dose-dependent inhibitory effect(all P<0.05).Conclusion:Edaravone inhibits IBMX-induced melanogenesis in B16 cells,and its mechanism of action may be related to the regulation of tyrosinase activity and HMB-45 expression.关键词
依达拉奉/酪氨酸酶/黑色素/黑色素瘤细胞B16/3-异丁基-1甲基黄嘌呤Key words
edaravone/tyrosinase/melanin/B16 cells/3-isobutyl-1-methylxanthine分类
临床医学引用本文复制引用
彭立红,吴泳宣,凌春桃,唐世杰..依达拉奉对3-异丁基-1-甲基黄嘌呤诱导的B16细胞黑色素生成的影响[J].汕头大学医学院学报,2025,38(1):1-5,5.基金项目
汕头市科技计划(汕府科[2022]169-65) （汕府科[2022]169-65）
