同位素2025，Vol.38Issue(2)：133-140,8.DOI:10.7538/tws.2024.youxian.106
99mTc-HYNIC-Duramycin的制备及凋亡显像研究
Preparation and Apoptosis Imaging Study of 99mTc-HYNIC-Duramycin
摘要
Abstract
Phosphatidylethanolamine(PE)is externalized to the cell surface during the early stages of apoptosis.Duramycin is a peptide that can bind to PE with high selectivity and specificity.Radionuclide labeled Duramycin enables in vivo imaging to monitor apoptosis at an early stage.In this study,the imaging agent 99mTc-HYNIC-Duramycin was obtained by one-step reaction between 99mTc and the precursor HYNIC-Duramycin.The radiolabeling process takes approximately 20 min,and the radiochemical purity is above 95%.99mTc-HYNIC-Duramycin is water-soluble,with a LogP value of-1.73±0.06(n=3),and the radiochemical purity remains above 95%in saline for up to 6 h,indicating excellent stability in vitro.Biodistribution studies showed that 99mTc-HYNIC-Duramycin is primarily metabolized by the liver,with higher radioactive accumulation observed in the kidneys and spleen,and lower uptake in blood and muscle.Compared with the control group and the blank group,99mTc-HYNIC-Duramycin has significantly higher uptake in NCI-H460 tumor after cisplatin treatment by SPECT/CT imaging.These results demonstrate the good specificity of the imaging agent and its ability to monitor chemotherapy-induced apoptosis in tumors at an early stage.In conclusion,99mTc-HYNIC-Duramycin is a promising SPECT/CT radiotracer for early treatment evaluation following chemotherapy.关键词
细胞凋亡/99mTc-HYNIC-Duramycin/SPECT/CT显像/疗效评估Key words
apoptosis/99mTc-HYNIC-Duramycin/SPECT/CT imaging/therapeutic evaluation分类
核科学引用本文复制引用
邱珊珊,罗田伟,高菲,孙明月,王宁,陈欢,陈孟毅,李洪玉,黄旭虎..99mTc-HYNIC-Duramycin的制备及凋亡显像研究[J].同位素,2025,38(2):133-140,8.基金项目
中核集团青年英才计划资助项目(20191216JJ-GK005) （20191216JJ-GK005）
