新疆师范大学学报（自然科学版）2025，Vol.44Issue(3)：23-28,6.
恩他卡朋联合运动对肥胖小鼠下丘脑FTO及肝脏GLUT4表达的影响研究
Effects of Entacapone Synergistic Exercise on Hypothalamic FTO and Hepatic GLUT4 Expression in Obese Mice
摘要
Abstract
To explore the effect of entacapone synergistic exercise intervention on the expression of hypothalamus obesity-related gene(FTO)and liver glucose transporter(GLUT4)in obese mice.40 obese mice induced by high-fat diet were randomly divided into 4 groups,namely high-fat group(HFD),entacapone group(ENT),exercise training group(EX)and entacapone+exercise training group(ENT-EX),and another 10 normal mice of the same age were used as blank control(CON).After 5 weeks of the experiment,the changes of fasting blood glucose in the mice were detected,Real-time PCR and Western blot were used to detect the expression of FTO in the hypothalamus and the expression of GLUT4 gene and protein in the liver,and the m6 A methylation kit was used to detect the m6 A methylation level in the hypothalamus.The results showed that,compared with the HFD group,the fasting blood glucose,FTO mRNA and protein expressions in the ENT-EX and EX groups were significantly decreased,and the GLUT4 mRNA and protein expressions were significantly increased,and the effect of the ENT-EX group was better than that of the EX group alone.There was no significant difference in m6 A methylation level in hypothalamus.The results showed that ENT-EX and EX interventions regulated the uptake and utilization of blood glucose by liver GLUT4 in obese mice to effectively reverse impaired glucose tolerance by inhibiting FTO expression in the hypothalamus.关键词
恩他卡朋/运动训练/肥胖小鼠/FTO表达/GLUT4表达Key words
Entacapone/Exercise/Obese mice/FTO expression/GLUT4 expression分类
生物学引用本文复制引用
韩贺龙,黄伟伟,任佳宝,何恩鹏,李艳红..恩他卡朋联合运动对肥胖小鼠下丘脑FTO及肝脏GLUT4表达的影响研究[J].新疆师范大学学报（自然科学版）,2025,44(3):23-28,6.基金项目
新疆维吾尔自治区自然科学基金面上项目(2022D01A100 （）
2021D01A120) （）
2023年新疆维吾尔自治区高校科研计划项目(XJEDU2023J031). （XJEDU2023J031）
