畜牧与兽医2025，Vol.57Issue(4)：8-13,6.
褪黑素改善绵羊冷冻卵母细胞损伤与发育潜能的研究
Melatonin alleviates the damage and improves the developmental potential of frozen ovine oocytes
摘要
Abstract
In order to determine the effect of melatonin(MLT)on the vitrification freezing of GV ovine oocytes,we selected the cumulus oocyte complex with more than three layers of dense intact cumulus cells and uniform cytoplasm,and added different concentrations(0,10-11,10-9,10-7,10-5 mol/L)of melatonin to the vitrification freezing and thawing solution.After thawing,the levels of reactive oxygen species(ROS),Ca2+and mitochondrial membrane potential(MMP)of oocytes cultured in vitro maturation medium for 24 hours were detec-ted.The results showed that,compared with the fresh group,the levels of ROS and Ca2+in the 0 mol/L MLT group were significantly in-creased(P＜0.05),and the level of MMP was significantly decreased(P＜0.05).Compared with the 0 mol/L MLT group,the addition of melatonin in vitrification freezing and thawing solution significantly reduced the ROS level and Ca2+concentration(P＜0.05),and signifi-cantly increased the MMP level of the oocytes(P＜0.05).The optimal melatonin concentration was 10-9 mol/L during vitrification of the GV stage oocytes.Compared with the 0 mol/L MLT group,the 10-9 mol/L group significantly increased the cleavage rate of oocytes(68.64%vs 58.03%,P＜0.05)and the blastocyst rate(18.97%vs 5.96%,P＜0.05).This study confirmed that melatonin supplementation in vitrifica-tion freezing and thawing solution alleviated GV stage ovine oocytes cryoinjury and improved their developmental potential.关键词
绵羊/卵母细胞/褪黑素/玻璃化Key words
sheep/oocytes/melatonin/vitrification分类
畜牧业引用本文复制引用
闫嘉欣,刘炳男,高子浩,张琳惠,李嘉,张慈,陶晨雨,李俊杰,夏威..褪黑素改善绵羊冷冻卵母细胞损伤与发育潜能的研究[J].畜牧与兽医,2025,57(4):8-13,6.基金项目
国家重点研发计划项目(2021YFD1200401) （2021YFD1200401）
河北省自然科学基金联合基金项目(C2023204007) （C2023204007）
河北省教育厅重点项目(ZD2022005) （ZD2022005）
河北省留学回国人才项目(ZD20220513) （ZD20220513）
河北农业大学人才引进专项项目(YJ2021013) （YJ2021013）
