畜牧与兽医2025，Vol.57Issue(4)：26-35,10.
山羊OR2AP1基因多态性及其与体尺性状的关联分析
Polymorphism of OR2AP1 gene in goats and its association with body size traits
摘要
Abstract
The aim of this study was to obtain one or more molecular selection markers and accelerate the process of goat selection and breeding with good growth performance and high meat production efficiency.Healthy adult ewes were selected for body size measurement and blood sample collection,the polymorphisms of OR2AP1 gene were detected by amplification of OR2AP1 gene,DNA sequencing and PCR-RFLP,the corresponding restriction enzymes were found by SnapGene software,and the unit point effect model constructed by IBM SPSS Statistics 26 was used to statistically analyze the goat sites to study the association between OR2AP1 gene polymorphisms and body size traits.The results showed that there were eight SNPs in the OR2AP1 gene:g.94G＞A,g.115G＞A,g.322C＞T,g.349C＞T,g.357C＞T,g.365T＞C,g.627G＞A and g.909C＞T.The loci affecting body height,body length and body oblique length were g.115G＞A,g.627G＞A,and g.627G＞A,respectively;and the loci affecting chest circumference,hind leg circumference,and tube circumference were g.115G＞A,g.＞357C＞T,g.357CT,and g.627G＞A,respectively;and g.115G＞A,g.357C＞T,and g.627G＞A,respectively;and g.115G＞A,g.357C＞T,g.365C＞T,respectively.The linkage disequilibrium analysis showed that the haplotype of the best combination of body size traits affecting goat were g.357C＞T and g.365T＞C:CCCT,TTTT;and g.115G＞A and g.357C＞T:AATT,GATT.The best combination haplotype of body height were CCCT and AATT,and the best combination haplotype of body length,chest circumference and jiri width were GATT,AATT and TTTT,respectively.In conclusion,g.115G＞A,g.357C＞T,and g.365C＞T and g.627G＞A could be used as molecular markers for improved goat body size traits,while CCCT,TTTT(g.357C＞T and g.365T＞C),AATT and GATT(g.115G＞A and g.357C＞T)were the dominant haplotype for breeding goats with excellent body size traits.关键词
SNP/遗传多样性/雷州山羊/川中黑山羊/努比亚山羊Key words
SNP/genetic diversity/Leizhou goat/Chuanzhong black goat/Nubian goat分类
畜牧业引用本文复制引用
符明永,李耀坤,赵秀慧,刘杰,董书餐,柳广斌,邓铭,郭勇庆,孙宝丽,刘德武..山羊OR2AP1基因多态性及其与体尺性状的关联分析[J].畜牧与兽医,2025,57(4):26-35,10.基金项目
广东省科技特派员项目(KTP20210344) （KTP20210344）
广东省现代农业产业技术体系项目(2023KJ127) （2023KJ127）
