畜牧与兽医2025，Vol.57Issue(4)：78-84,7.
猪萨佩罗病毒VP4蛋白多克隆抗体制备及功能验证
Preparation of polyclonal antibody against porcine saperovirus VP4 protein and its application
摘要
Abstract
In order to investigate porcine sapelovirus(PSV)in depth,this study utilized the SHCM2019 strain to prepare VP4 protein and its polyclonal antibodies through prokaryotic expression.Genetic evolutionary analysis was conducted based on the amino acid sequence of VP4.Then,the VP4 gene was ligated into the prokaryotic expression vector pET-N-GST-Precission and transformed.Induced expression resulted in soluble VP4 protein.Next,BALB/c mice were immunized with the purified VP4 protein to prepare polyclonal antibodies,which were subsequently validated for their reactivity using ELISA,Western blot,and IFA assays.The ELISA results revealed that the polyclonal antibody titre reached 1∶128 000,demonstrating high specificity.The Western blot and IFA results indicated that the antibody could specif-ically recognize and bind to PSV,suggesting that the recombinant VP4 protein possessed good immunogenicity and reactivity.This study suc-cessfully expressed the VP4 protein with a GST tag and produced polyclonal antibodies,laying the groundwork for future research on the func-tion and applications of the VP4 gene.关键词
猪萨佩罗病毒/VP4 蛋白/原核表达/多克隆抗体Key words
porcine saperovirus/VP4 protein/prokaryotic expression/polyclonal antibody分类
农业科技引用本文复制引用
钟祖昌,李本强,陶洁,程靖华,石迎,刘佩红,唐攀,焦嘉杰,刘惠莉..猪萨佩罗病毒VP4蛋白多克隆抗体制备及功能验证[J].畜牧与兽医,2025,57(4):78-84,7.基金项目
上海市农业科技创新项目(2023-02-08-00-12-F04612) （2023-02-08-00-12-F04612）
上海市农业科学院动物疾病防控卓越团项目(沪农科卓[2022]012) （沪农科卓[2022]012）
