畜牧与兽医2025，Vol.57Issue(4)：85-92,8.
1株犬冠状病毒山西株变异与遗传进化特征
Gene variation and genetic evolution characteristics of a canine coronavirus strain in Shanxi Province
摘要
Abstract
To elucidate the homology and genetic variation of a canine coronavirus(CCoV)strain in Shanxi Province,the present study amplified the S gene and the M gene of the CCoV Shanxi strain,analyzing the gene,amino acid homology and genetic evolution of the CCoV Shanxi strain using the DNASTAR,ESPript 3.x and MEGA-X softwares,respectively.The results showed that the S gene homology of the CCoV Shanxi strain with published reference sequences was88.6%-98.3%,and its M gene homology was 83.6%-96.3%.The S protein and M protein of the CCoV Shanxi strain had partial amino acid site variation,among which the S protein was mainly amino acid point muta-tion,and the M protein showed a continuous amino acid mutation region at sites of 10 to 40.The genetic evolutionary tree constructed using the Neighbor-Joining method showed that the CCoV Shanxi strain belonged to the CCoV type Ⅱ strain,which was in the same branch with that of the CCoV isolated in Vietnam(LC190907).In summary,The analysis results of the S gene and M gene were consistent,which indi-cated that the CCoV Shanxi strain belonged to CCoV type Ⅱ strain,with different degrees of gene and amino acid variation,and was closely related to the strain isolated in Vietnam.The findings of the present study provided an important theoretical reference for the study of CCoV etiology and disease control in Shanxi Province.关键词
犬冠状病毒/纤突蛋白基因/膜蛋白基因/同源性/遗传进化Key words
canine coronavirus/S gene/M gene/homology/genetic evolution分类
农业科技引用本文复制引用
刘晶英,李炜,杨勃,孙子龙,张鼎..1株犬冠状病毒山西株变异与遗传进化特征[J].畜牧与兽医,2025,57(4):85-92,8.基金项目
山西省回国留学人员科研资助项目(2023-094) （2023-094）
