畜牧与兽医2025，Vol.57Issue(4)：93-100,8.
谷氨酸脱羧酶lmo2363基因缺失对单增李斯特菌致病性的影响
Effect of deletion of glutamate decarboxylase lmo2363 gene on pathogenicity of Listeria monocytogenes
摘要
Abstract
The aim of this study was to investigate the effect of the deletion of glutamate decarboxylase lmo2363 gene on the pathogenicity of Listeria monocytogenes.In here,the reference strain of Listeria monocytogenes ATCC 19111 was used as the control.The biofilm formation of LM83-1,LM83-1Δlmo2363 and CLM83-1Δlmo2363 was observed by fluorescence microscopy.Caco-2 cells and RAW264.7 cells were used for adhesion,invasion and intracellular proliferation tests of the above bacterium.Transcriptome sequencing of LM83-1 and LM83-1Δlmo2363 was performed to screen the differentially expressed genes between them,and GO functional enrichment analysis and KEGG met-abolic pathway enrichment analysis were performed for differentially expressed genes.The results showed that,compared with LM83-1,the biofilm structure of LM83-1Δlmo2363 decreased and the biofilm formation of CLM83-1Δlmo2363 was the same as that of LM83-1 at 24 h and 48 h.The adhesion rate of LM83-1Δlmo2363 to Caco-2 cells was enhanced,and the invasion rate was decreased.The adhesion rate and invasion rate of CLM83-1Δlmo2363 to Caco-2 cells were not significantly different from those of LM83-1.The proliferation of LM83-1Δlmo2363 in macrophages decreased at 4,8,12 and 24 h,and the proliferation of CLM83-1Δlmo2363 in macrophages decreased at 4 h.Transcriptome sequencing showed that 136 genes of the deletion strain LM83-1Δlmo2363 were significantly different from those of LM83-1,including 36 up-regulated genes and 100 down-regulated genes.The GO functional enrichment analysis showed that the differentially ex-pressed genes were mainly concentrated in GO items such as ethanolamine lysozyme activity,bacterial flagella substrate,ethanolamine catab-olism,iron ion homeostasis,etc.The enrichment analysis of the KEGG metabolic pathways showed that the differentially expressed genes were mainly concentrated in the phosphotransferase system,starch and sucrose metabolism,and flagella assembly.These results suggested that the glutamate decarboxylase lmo2363 gene may played an important role in the formation of LM biofilm and in vitro cell infection by par-ticipating in biological processes such as iron homeostasis,flagella assembly and phosphotransferase system;which laid a foundation for fur-ther exploration into the function of glutamate decarboxylase.关键词
单增李斯特菌/谷氨酸脱羧酶/生物被膜/体外细胞感染/转录组测序Key words
Listeria monocytogenes/glutamic acid decarboxylase/biofilm/in vitro cell infection/transcriptome sequencing分类
农业科技引用本文复制引用
芝吉,张坤中,崇倩,薛惠文,苟惠天,曹青,赵学慧,张浩浩,范子秋,马永辉,邓静,何曾文,马金锐..谷氨酸脱羧酶lmo2363基因缺失对单增李斯特菌致病性的影响[J].畜牧与兽医,2025,57(4):93-100,8.基金项目
国家自然科学基金项目(31960726,32060822,31560700) （31960726,32060822,31560700）
国家重点研发计划项目(2019YFC1605705) （2019YFC1605705）
甘肃农业大学青年导师扶持基金项目(GAU-QDFC-2020-10) （GAU-QDFC-2020-10）
甘肃省重点研发计划项目(20YF8FA136) （20YF8FA136）
张家川揭榜挂帅项目(ZC-STK-2023A-030) （ZC-STK-2023A-030）
