畜牧与兽医2025，Vol.57Issue(4)：101-106,6.
非洲猪瘟病毒CP312R蛋白单克隆抗体的制备与鉴定
Generation and characterization of monoclonal antibodies against CP312R protein of African swine fever virus
摘要
Abstract
To deeply study the biological characteristics of CP312R protein and provide basis for serological diagnostic technology research and vaccine development,monoclonal antibodies of African swine fever virus(ASFV)CP312R protein were prepared.The prokaryotic ex-pression plasmid pGEX-6p-CP312R was transformed into E.coli BL21(DE3)cells and purified after inducing expression with IPTG.The antigenicity of the recombinant protein was determined by SDS-PAGE and Western blot analysis.Then,BALB/c mice were immunized with the purified protein.After cell fusion and positive clone screening,two positive hybridoma cells that could stably secrete CP312R monoclonal antibodies were obtained.The Western blot results showed that two monoclonal antibodies were able to react specifically with the CP312R pro-tein.The IFA results showed that both monoclonal antibodies produced specific green fluorescence.The two monoclonal antibodies prepared in this study laid the foundation for the study of the serological diagnostic method and vaccine of ASFV.关键词
非洲猪瘟病毒/CP312R蛋白/单克隆抗体/原核表达Key words
African swine fever virus/CP312R protein/monoclonal antibody/prokaryotic expression分类
农业科技引用本文复制引用
司安琪,王淑娟,张永强,王筱真,任炜杰,郑龙三,包静月,王志亮..非洲猪瘟病毒CP312R蛋白单克隆抗体的制备与鉴定[J].畜牧与兽医,2025,57(4):101-106,6.基金项目
国家重点研发计划项目(2022YFD1800500) （2022YFD1800500）
