新医学2025，Vol.56Issue(3)：268-274,7.DOI:10.12464/j.issn.0253-9802.2024-0462
miR-223-3p通过靶向NLRP3恢复高血压脑出血大鼠的认知功能
miR-223-3p recover cognitive function in rats with hypertensive intracerebral hemorrhage by targeting NLRP3
摘要
Abstract
Objective To explore the effects of miR-223-3p on cognitive function recovery in rats with hypertensive intracerebral hemorrhage(HICH)and its regulatory mechanisms.Methods The rat model of HICH was constructed to analyze changes in miR-223-3p expression and cognitive function.Subsequently,miR-223-3p was overexpressed or knocked down to investigate its effects on the expression of the inflammasome NLRP3 and cognitive function in rats.NLRP3 was then knocked down to confirm its role in cognitive function in HICH rats.Finally,a luciferase assay was performed to identify the direct targeting effect of miR-223-3p on NLRP3.Results The expression of miR-223-3p was upregulated in the brain tissues of HICH rats.miR-223-3p inhibits NLRP3 expression and recovers cognitive function in rats.The luciferase assay demonstrated that miR-223-3p could directly target the mRNA of NLRP3 and downregulate its expression.Conclusion miR-223-3p downregulates NLRP3 expression by binding to its mRNA,thereby promoting the recovery of cognitive function in rats with intracerebral hemorrhage.关键词
miR-223-3p/炎症小体/NLRP3/高血压/脑出血/大鼠Key words
miR-223-3p/Inflammasome/NLRP3/Hypertension/Intracerebral hemorrhage/Rats引用本文复制引用
余梦擎,吴金波,赖志勇,陈忠强..miR-223-3p通过靶向NLRP3恢复高血压脑出血大鼠的认知功能[J].新医学,2025,56(3):268-274,7.基金项目
广东省医学科研基金-青年项目(A2023578) （A2023578）
