畜牧与兽医2025，Vol.57Issue(4)：121-129,9.
羊轮状病毒GS2023株NSP2基因克隆及序列分析
Cloning and sequence analysis of NSP2 gene of sheep rotavirus GS2023 strain
摘要
Abstract
In order to study the structure and function of sheep rotavirus NSP2 protein,the cDNA of a sheep rotavirus GS2023 strain was used as a template to amplify and clone the NSP2 gene by PCR,and various bioinformatics analyses and prediction software were used to ana-lyze the bioinformatics characteristics of NSP2.The results showed that the open reading frame of the NSP2 gene was 954 bp,encoding 317 amino acids.NSP2 was a hydrophilic and stable protein,with no signal peptide.The prediction of subcellular localization showed that it was mainly located in cytoplasm and nucleus and belonged to non-transmembrane protein.The NSP2 protein contained 36 glycosylation sites and 49 phosphorylation sites.Its secondary structure was composed of α helix structure,extended chain and random curl,while its tertiary struc-ture was connected end to end.The amino acid similarity between the NSP2 protein and the rotavirus strains in China and in the world was 90.2%-98.7%.Compared with bovine rotavirus strains SCMY1 and SCMY2 in China,the amino acids of GS2023 were mutated from lysine to arginine at position 38,and from serine to asparagine and from valine to isoleucine at positions 100 and 202,respectively.The phylogenet-ic tree analysis showed that the genetic distance between the GS2023 strain and the bovine rotavirus strains in China was the closest,but it was far from other sheep rotaviruses.In this study,the NSP2 gene of a sheep rotavirus was successfully cloned and the biological characteris-tics of the NSP2 protein were analyzed,which provided theoretical reference for further research on the structure and function of the NSP2 protein.关键词
羊/轮状病毒/NSP2/序列分析/系统发育进化分析Key words
sheep/rotavirus/NSP2/sequence analysis/phylogenetic analysis分类
农业科技引用本文复制引用
于頔浠,李家奎,赵登率,张远航,李平,王天宇,张克山,高寒,覃丽梅,赵孟孟..羊轮状病毒GS2023株NSP2基因克隆及序列分析[J].畜牧与兽医,2025,57(4):121-129,9.基金项目
国家重点研发计划项目(2023YFD1801302,2023YFD1801301) （2023YFD1801302,2023YFD1801301）
国家肉牛牦牛产业技术体系项目(CARS-37) （CARS-37）
佛山大学高层次人才研究创业项目(CGZ07001) （CGZ07001）
