针刺研究2025，Vol.50Issue(3)：260-269,10.DOI:10.13702/j.1000-0607.20240550
艾灸对血管性痴呆大鼠认知功能及海马线粒体动力学相关蛋白的影响
Effect of moxibustion on cognitive function and hippocampal mitochondrial dynamics related proteins in rats with vascular dementia
摘要
Abstract
Objective To investigate the effect of moxibustion on hippocampal mitochondrial dynamics-related proteins in rats with vascular dementia(VD),so as to reveal the underlying mechanisms of moxibustion in treating VD.Methods SD rats were randomly divided into sham operation,model,moxibustion and medication groups,with 12 rats in each group.The VD model was prepared using an improved bilateral common carotid artery ligation method.Mild moxibstion was applied to"Shenting"(GV24),"Baihui"(GV20)and"Dazhui"(GV14)for 20 min,once daily for 6 days per week.Rats of the medication group were treated with oral administration of nimodipine(12 mg/kg)once daily.All above interventions were performed for 4 weeks.Learning and memory abilities were assessed using Morris water maze test.Histopathological changes of hippocampus was observed with HE staining.Mitochondrial membrane potential(Δψm)and reactive oxygen species(ROS)levels in hippocampal tissue were measured by flow cytometry.The mRNA and protein expression levels of optic atrophy protein 1(Opa1),mitochondrial fusion protein 1(Mfn1),mitochondrial fusion protein 2(Mfn2),dynamics-related protein 1(Drp1),fission protein 1(Fis1),mitochondrial fission factor(Mff)in hippocampus was detected by PCR or Western blot,respectively.Results Compared with the sham operation group,the escape latency was prolonged(P<0.01),the times of crossing the original platform were reduced(P<0.01),the membrane potential of hippocampus,the mRNA and protein expression levels of Opa1,Mfn1 and Mfn2 in hippocampus were decreased(P<0.01,P<0.001)in the model group,while the average fluorescence intensity of ROS was increased,the mRNA and protein expression levels of Drp1,Fis1 and Mff were increased(P<0.001).In comparison with the model group,the escape latency was significantly shortened(P<0.05),the times of crossing the original platform were increased(P<0.05),the membrane potential of the hippocampus,the mRNA and protein expression levels of Opa1,Mfn1 and Mfn2 were increased(P<0.05,P<0.001)in the moxibustion and medication groups,while the average fluorescence intensity of ROS in the hippocampus,the mRNA and protein expression levels of Drp1,Fis1 and Mff were decreased(P<0.001).The average fluorescence intensity of ROS in the moxibustion group was significantly lower than that in the medication group(P<0.001).HE staining showed loose arrangement of neurons,disappearance of partial nucleolus,and necrocytosis after modeling,which were relatively milder in both moxibustion and medication groups.Conclusion Moxibustion can effectively improve the cognitive function of VD rats,and its mechanism may be related to regulating hippocampal mitochondrial dynamics-related proteins,promoting mitochondrial fusion,inhibiting excessive mitochondrial division,thereby improving the imbalance of mitochondrial dynamics and alleviating mitochondrial dysfunction-related conditions.关键词
血管性痴呆/艾灸/线粒体/线粒体动力学/融合与分裂Key words
Vascular dementia/Moxibustion/Mitochondria/Mitochondrial dynamics/Fusion and fission引用本文复制引用
杨琪琪,杨骏,叶敏,王克坡,周欣华,李蔚然,李飞..艾灸对血管性痴呆大鼠认知功能及海马线粒体动力学相关蛋白的影响[J].针刺研究,2025,50(3):260-269,10.基金项目
国家中医药管理局青年岐黄学者支持项目(No.国中医药人教函[2022]256号) （No.国中医药人教函[2022]256号）
第九批安徽省特支计划人才项目(No.皖组办字[2023]35号) （No.皖组办字[2023]35号）
安徽高校自然科学重点研究项目(No.KJ2021A0549) （No.KJ2021A0549）
