针刺研究2025，Vol.50Issue(3)：295-301,7.DOI:10.13702/j.1000-0607.20231124
基于IL-6/STAT3信号通路探讨线香灸对急性湿疹大鼠Th17/Treg平衡的调节机制
Joss-stick moxibustion reduces inflammatory response of acute eczema by inhibiting IL-6/STAT3 signaling,and balancing Th17/Treg to normalize filaggrin and Caspase-14 levels in rats
摘要
Abstract
Objective To investigate the effect of joss-stick moxibustion on the balance of helper T cell 17(Th17)/regulatory T cell(Treg)through interleukin-6(IL-6)/signal transduction and transcriptional activator 3(STAT3)pathway in acute eczema(AE)rats.Methods A total of 24 male SD rats were randomly divided into blank control,AE model,boric acid solution(BAS)washing,and moxibustion groups,with 6 rats in each group.The AE model was established by external application of 7%dinitrochlorobenzene(DNCB,100 μL)to the abdominal skin once for the first day,3 days later,the 5%DNCB to the right back sin,every three days for 3 times.Rats of the blank control group received external application of distilled water at the skin and those of the BAS washing group received external application of 3%BAS solution wet packing.Rats of the moxibustion group received local heat stimulation of joss-stick moxibustion at the injured skin locus for 30 min,once a day,5 days a week,for 2 weeks.At the end of the experiments,the injured skin(on the right side of the back)and the healthy skin on the left side of the back were sampled using a round puncher(6 mm in diameter)for calculating the skin swelling degree.The inhibition rate equals to the skin swelling degree/weight of the healthy skin×100%.The content of serum IL-6 was detected using ELISA,the expression levels of STAT3 and ROR-γt mRNAs in the injured skin tissue were detected using real-time fluorescence quantitative PCR,and the expression levels of p-STAT3,ROR-γt,and Foxp3 protein were detected using Western blot.The immunoactivities of filaggrin(FLG)and Caspase-14 in the injured skin tissue were detected by immunohistochemistry.Results Compared with the blank control group,the topical skin swelling degree,inhibition rate,serum IL-6 content,expression levels of STAT3 and ROR-γt mRNAs,and p-STAT3,ROR-γt protein expression levels were significantly increased(P<0.01,P<0.05),while the expression level of Foxp3 protein and the immunoactivities of FLG and Caspase-14 were markedly down-regulated(P<0.01)in the model group.In comparison with the model group,both the increase and decrease of the aforementioned 10 indexes after the modeling were reversed in the BAS washing and moxibustion groups(P<0.01,P<0.05).No significant differences were found between the BAS washing and moxibustion groups in the abovementioned 10 indexes.Conclusion Joss-stick moxibustion can reduce the inflammatory response in AE rats,which may be related to its functions in suppressing the overexpression of IL-6/STAT3 signaling pathway to promote the restoration of Th17/Treg balance,thus normalizing the levels of FLG and Caspase-14.关键词
急性湿疹/线香灸/白细胞介素-6/信号传导及转录激活因子3/辅助性T细胞17/调节性T细胞Key words
Acute eczema/Joss-stick moxibustion/IL-6/STAT3/Th17/Treg引用本文复制引用
李欣莹,赵荧荧,李志杰,龙慧欣,汤晓艳,李浩天,周子阳,韩兴军..基于IL-6/STAT3信号通路探讨线香灸对急性湿疹大鼠Th17/Treg平衡的调节机制[J].针刺研究,2025,50(3):295-301,7.基金项目
济南市科技计划项目(No.202019002) （No.202019002）
山东省医药卫生科技发展计划项目(No.202204120705) （No.202204120705）
山东省中医药科技项目(No.Z-2023006T) （No.Z-2023006T）
