针刺研究2025，Vol.50Issue(3)：319-326,8.DOI:10.13702/j.1000-0607.20240144
灸药联合对乳腺癌荷瘤小鼠肿瘤组织免疫检查点的影响
Effect of moxibustion combined with chemotherapy on immune checkpoints in tumor tissue of breast cancer-bearing mice
摘要
Abstract
Objective To observe the effects of moxibustion combined with chemotherapy on immune checkpoints including programmed cell death protein 1(PD-1),T cell immunoglobulin domain and mucin domain(TIM-3)and cytotoxic T-lymphocyte-associated protein 4(CTLA-4)in the tumor tissue of breast cancer-bearing mice,so as to explore the effect and mechanisms of moxibustion combined with chemotherapy on breast cancer.Methods Forty BALB/c female mice were randomly divided into model,chemotherapy,moxibustion and combination groups,with 10 mice in each group.4T1 tumor cells were inoculated into the fat pad under the left fourth nipple of the mice to establish the breast cancer-bearing mice model.The chemotherapy group was injected intraperitoneally with doxorubicin(DOX)solution at 2.5 mg/kg once every 3 days;the moxibustion group received bilateral"Zusanli"(ST36)moxibustion with 2 moxa cones per point,treated every 2 days;the combination group received both DOX injection and moxibustion intervention.The above interventions lasted for 21 days.The body weight and tumor volume of the mice were recorded daily.HE staining was used to observe tumor tissue pathological morphology.Immunohistochemistry and Western blot were used to detect the positive expression and protein expression levels of PD-1,TIM-3,and CTLA-4 in the tumor tissue.Results Compared with the model group after intervention,the body weight was increased(P<0.01,P<0.05),the tumor volume was decreased(P<0.01),the pathological morphology of tumor tissue showed varying degrees of tumor cells degeneration,and the positive expression and protein expression levels of PD-1,TIM-3,and CTLA-4 in tumor tissue were decreased(P<0.01,P<0.05)of mice in the chemotherapy,moxibustion and combination groups.Compared with the chemotherapy group,the body weight of mice in the moxibustion group was higher(P<0.01);the tumor volume of mice in the combination group was smaller(P<0.01);the positive expression and protein expression levels of PD-1,TIM-3,and CTLA-4 were further reduced(P<0.01,P<0.05)of mice in the moxibustion and combination groups.Compared with the moxibustion group,the body weight was decreased(P<0.01),the tumor volume was smaller(P<0.01),the degree of tumor cell degeneration was higher,and the positive expression and protein expression levels of PD-1,TIM-3,and CTLA-4 were decreased(P<0.01)of mice in the combination group.Conclusion Moxibustion combined with chemotherapy shows more significant effects in inhibiting tumor growth in breast cancer-bearing mice compared to moxibustion or chemotherapy alone.It reduces the expression of PD-1,TIM-3,and CTLA-4 in tumor tissues,suggesting that lowering immune checkpoint expression levels may be one of the mechanisms by which moxibustion combined with chemotherapy drugs treats tumors.关键词
艾灸/乳腺癌/免疫检查点/程序性死亡蛋白1/T细胞免疫球蛋白黏蛋白-3/细胞毒性T淋巴细胞相关蛋白4Key words
Moxibustion/Breast cancer/Immune checkpoints/Programmed cell death protein 1(PD-1)/T cell immunoglobulin domain and mucin domain(TIM-3)/Cytotoxic T-lymphocyte-associated protein 4(CTLA-4)引用本文复制引用
张飞程,高田宇,张晨曦,李继娟,梁新月,张晓琪,贾春生,潘丽佳..灸药联合对乳腺癌荷瘤小鼠肿瘤组织免疫检查点的影响[J].针刺研究,2025,50(3):319-326,8.基金项目
河北中医药大学2022年博士科研基金项目(No.BSZ2022006) （No.BSZ2022006）
河北省自然科学基金项目(No.H2023423029) （No.H2023423029）
