液晶与显示2025，Vol.40Issue(4)：588-597,10.DOI:10.37188/CJLCD.2024-0327
基于溶致液晶制备拉曼光谱增强基底的新冠病毒检测
Detection of NeoCoronavirus based on Raman spectroscopy-enhanced substrates prepared by lyotropic liquid crystals
摘要
Abstract
Surface-enhanced Raman spectroscopy(SERS)substrates of silver nanoflowers were prepared based on lyotropic liquid crystals for the quantitative detection of neoCoronavirus spike protein(S protein).Firstly,a Raman spectroscopy detection scheme for neoCoronavirus was designed,and the SERS substrate was prepared using lyotropic liquid crystal soft templates and electrochemical deposition techniques.Then,spectral consistency and inter-substrate detection differences were evaluated to ensure reliable quantitative detection.Then,the sensitivity of the SERS substrate for the detection of S protein was analysed,and its detection limit was 2×10-6 mg/mL.Finally,the quantitative detection method was established,yielding a precise concentration-intensity relationship with a measurement error of 4×10-7 mg/mL.In this paper,the technique of preparing SERS substrates based on lyotropic liquid crystals was used for the high sensitivity detection of neoCoronavirus spike protein,and also provides a feasible technical way for the detection of other proteins.关键词
溶致液晶/表面增强拉曼散射/拉曼光谱/新冠病毒刺突蛋白/高灵敏检测Key words
lyotropic liquid crystals/surface-enhanced Raman scattering/Raman spectroscopy/NeoCoronavirus spike protein/high sensitivity detection引用本文复制引用
叶佳伟,唐一博,李科良,宋从喜,徐浩然,毛红敏,陆焕钧,曹召良..基于溶致液晶制备拉曼光谱增强基底的新冠病毒检测[J].液晶与显示,2025,40(4):588-597,10.基金项目
江苏省"十四五"重点学科(No.2021135) （No.2021135）
国家自然科学基金(No.22205155) （No.22205155）
江苏省自然科学基金(No.BK20220640) （No.BK20220640）
江苏省高等学校自然科学基金(No.22KJB150011) （No.22KJB150011）
江苏省科协青年精英科学家资助计划(No.JSTJ-2023-XH042)Supported by Jiangsu Key Disciplines of the Fourteenth Five-Year Plan(No.2021135) （No.JSTJ-2023-XH042）
National Natural Science Foundation of China(No.22205155) （No.22205155）
Natural Science Foundation of Jiangsu Province(No.BK20220640) （No.BK20220640）
Natural Science Foundation of Jiangsu Higher Education Institutions of China(No.22KJB150011) （No.22KJB150011）
Young Elite Scientists Sponsorship Program by Jiangsu Association for Science and Technology(No.JSTJ-2023-XH042) （No.JSTJ-2023-XH042）
