云南民族大学学报（自然科学版）2025，Vol.34Issue(2)：128-132,143,6.DOI:10.3969/j.issn.1672-8513.2025.02.002
黄毛耳草不同部位香豆素、黄酮质量分数测定及抗氧化活性研究
Content determination of coumarin and flavone in different parts of Hedyotis chrysotricha and their antioxidant activity
摘要
Abstract
Based on high-performance liquid chromatography and the 1,1-diphenyl-2-trinitrophenylhydrazine 2,2'-azino-bis(3-ethylbenzo thiazoline-6-sulfonic acid)diammonium salt,ferric reduction/antioxidant capacity methods were used for the simultaneous determination of scopolin,rutin,kaempferol-3-O-rhamnoside and coumarins,total flavonoids content in different parts of Eupatorium adenophorum and evaluation of antioxidant activity in different parts.The results showed that contents of scopolin,rutin,kaempferol-3-O-rhamnoside,total coumarins and total flavonoids were found in the leaves,followed by whole grass,with the lowest content in the stems.The antioxidant capacity was:leaf>whole grass>stems.The established method is simple,accurate and repeatable,making it suitable for the quantitative analysis of components and evaluation of pharmacological activity in different parts of Eupatorium adenophorum.关键词
黄毛耳草/茎/叶/总香豆素/总黄酮/抗氧化Key words
Hedyotis chrysotricha/stem/leaves/total coumarins/total flavonoids/scopolamine lactone分类
医药卫生引用本文复制引用
孙宗淼,许秀萍,金贤武..黄毛耳草不同部位香豆素、黄酮质量分数测定及抗氧化活性研究[J].云南民族大学学报（自然科学版）,2025,34(2):128-132,143,6.基金项目
江西省卫健委中医药科技课题(SZYYB20225082). （SZYYB20225082）
