云南民族大学学报（自然科学版）2025，Vol.34Issue(2)：133-143,11.DOI:10.3969/j.issn.1672-8513.2025.02.003
阔叶十大功劳治疗肝癌作用机制的生物信息学研究
Bioinformatics study on the mechanism of Mahonia bealei(Fort.)Carr in the treatment of liver cancer
摘要
Abstract
The therapeutic mechanisms of Mahonia bealei(Fort.)Carr(MB)in the treatment of liver cancer are explored based on network pharmacology and bioinformatics.First,the targets of MB were obtained from the Traditional Chinese Medicine Systems Pharmacology(TCMSP)database,and the liver cancer dataset GSE45267 was downloaded from the GEO database.A protein-protein interaction network was constructed using Cytoscape software and the STRING database,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses through the DAVID database.Additionally,differential expression,protein analysis,clinical relevance,and survival analyses were conducted using the GEPIA,THPA,UALCAN,and Kaplan-Meier databases.Molecular docking was performed using DS BIOVIA Discovery Studio 2016 v16.1 software.The study identified eight hub genes(CCNB1,CCNB2,AURKA,CCNA2,CCNE1,CHEK1,CDK1,TOP2A),which were significantly overexpressed in liver cancer tissues.Survival analysis revealed that these eight hub genes were significantly associated with overall survival and progression-free survival.Molecular docking results indicated that seven proteins(CCNB1,AURKA,CCNA2,CCNE1,CHEK1,CDK1,TOP2A)could interact with MB components.Overall,network pharmacology and bioinformatics analyses suggest that MB may exert therapeutic effects on liver cancer through multiple components,targets,and pathways.关键词
网络药理学/生物信息学/阔叶十大功劳/肝癌/作用机制Key words
network pharmacology/bioinformatics/liver cancer/Mahonia bealei(Fort.)Carr/mechanism of action分类
医药卫生引用本文复制引用
杨霞,杨凯平,张学武,林登梅,张楠楠,李军..阔叶十大功劳治疗肝癌作用机制的生物信息学研究[J].云南民族大学学报（自然科学版）,2025,34(2):133-143,11.基金项目
国家自然科学基金(82060797) （82060797）
贵州中医药大学大学生创新创业训练计划项目(贵中医大创合字(2022)135号). （贵中医大创合字(2022）
