中国免疫学杂志2025，Vol.41Issue(3)：529-534,6.DOI:10.3969/j.issn.1000-484X.2025.03.003
敲除ST2表达对ConA诱导的自身免疫性肝炎小鼠肠道菌群的影响
Effect of knockout ST2 expression on intestinal microflora in mice with ConA-induced autoimmune hepatitis
摘要
Abstract
Objective:To investigate the effect of growth stimulation expressed gene 2(ST2)on intestinal microflora of mice with ConA-induced autoimmune hepatitis(AIH)by knockout.Methods:ST2 gene knockout mice were constructed by gene knockout technique.On this basis,AIH mouse model was established by ConA induction.The relative expression of cytokines was detected by real-time fluorescence quantitative PCR(RT-PCR).Intestinal microorganisms were sequenced using the 16S rRNA high-through put sequencing method,and the sequencing results were analyzed using Microbial Ecology related software and database.Results:Com-pared with the control group,ALT and AST were decreased,the damage of liver was mild,and the relative expression of IL-6 was also decreased in the liver of mice with ConA-induced AIH after ST2 gene knockout.There was no significant difference in the ACE and CHAO indices of the abundance of reactive flora.There was no significant difference between Shannon index and Simpson index re-flecting bacterial diversity.Psychrobacter,unclassified_Clostridia,Halothiobacillus,Clostridium_XlVa and Haemophilus genus content increased;Romboutsia,Rikenella,Parabacteroides and unclassified_Clostridiales bacterial content reduce.The areas under the receiv-er operator characteristic(ROC)curves were 0.88,0.89,0.88,0.80,0.90,0.90,0.84,0.91 and 0.84,respectively.Conclusion:Knockout of ST2 gene expression in mice with AIH can reduce liver injury and inflammation,and does not affect the distribution abun-dance and diversity of intestinal flora.However,there are differences among bacteria genera,and it has good diagnostic value.关键词
ST2敲除小鼠/刀豆蛋白A/肠道菌群/自身免疫性肝炎/诊断价值Key words
ST2 knockout mice/ConA/Intestinal flora/Autoimmune hepatitis/Diagnostic value分类
医药卫生引用本文复制引用
江仁权,汤纪丰,何毓珏,夏锦芳,林锦骠,欧启水..敲除ST2表达对ConA诱导的自身免疫性肝炎小鼠肠道菌群的影响[J].中国免疫学杂志,2025,41(3):529-534,6.基金项目
国家自然科学基金项目(82072356) （82072356）
福建省卫生健康青年科研课题(2021QNA030). （2021QNA030）
