中国免疫学杂志2025，Vol.41Issue(3)：535-539,5.DOI:10.3969/j.issn.1000-484X.2025.03.004
GBP3依赖其GTP酶活性负向调控HTLV-1病毒复制
GBP3 negatively regulates HTLV-1 replication in a GTPase-dependent manner
摘要
Abstract
Objective:To investigate the effect of GBP3 on replication of adult T-lymphocytic leukemia virus type 1(HTLV-1).Methods:Expression of GBP3 in HTLV-1-infected HeLa cell and THP1 cell was detected by Western blot.The knock-down efficiency of siRNAs targeting GBP3 in HeLa and THP1 cells was evaluated by Western blot.Effects of GBP3 overexpression or knockdown on expression of HTLV-1 proviral transcripts Tax,px,HBZ,Gag,ENV,5'UTR,and viral proteins Tax,p19 were investigated by RT-qPCR and Western blot.GTPase-defective mutant of GBP3,GBP3K51A was constructed to explore whether the effects of GBP3 on HTLV-1 infection were dependent on its GTPase activity.Results:GBP3 expression was upregulated in HTLV-1 infected HeLa and THP1 cells.GBP3 overexpression decreased expression of HTLV-1 proviral transcripts and viral proteins,whereas the knockdown of GBP3 has the opposite effects.Overexpression of GBP3K51A increased expression of HTLV-1 proviral transcripts and viral proteins.Conclusion:HTLV-1 virus infection can induce expression of GBP3;overexpression of GBP3 inhibits virus replication and may depend on GTPase.关键词
GBP3/HTLV-1/抗病毒/GTP酶活性Key words
GBP3/HTLV-1/Antivirus/GTPase分类
医药卫生引用本文复制引用
刘燕子,赵鑫,秦潇,黄雨璐,杨曦,范晴晴,杨波,王洁..GBP3依赖其GTP酶活性负向调控HTLV-1病毒复制[J].中国免疫学杂志,2025,41(3):535-539,5.基金项目
国家自然科学基金项目(32070949). （32070949）
