中国免疫学杂志2025，Vol.41Issue(3)：545-550,6.DOI:10.3969/j.issn.1000-484X.2025.03.006
梓醇调节TLR4/NF-κB信号通路对急性乙型肝炎大鼠肝损伤的保护作用研究
Protective effect of catalpol on liver injury in acute hepatitis B rats by regulating TLR4/NF-κB signaling pathway
摘要
Abstract
Objective:To investigate the protective effect of catalpol on the liver of rats with acute hepatitis caused by hepatitis B virus(HBV)infection and its possible mechanism.Methods:SD rats were randomly divided into Sham group,Model group,acyclo-vir group(ACY group),low-dose catalpol group(L-CAT group),medium-dose catalpol group(M-CAT group)and high-dose catalpol group(H-CAT group).The levels of serum inflammatory cytokines were detected by ELISA.Flow cytometry was used to detect the per-centage of peripheral blood Th17 cells.Hematoxylin-eosin staining and TUNEL assay were used to detect liver tissue injury and apopto-sis.Western blot analysis was used to detect the expression of related proteins in liver tissue.Results:Compared with the Sham group,the levels of serum HBsAg,HBeAg,ALT,AST,Th17 cell ratio,IL-17 and IL-23,and the expression of TLR4 and p-p65 protein in liver tissues were significantly increased in the Model group,while the levels of serum IL-13 and IL-4 were significantly decreased in the Model group,the differences were statistically significant(P<0.05).Compared with the Model group,the levels of serum HBsAg,HBeAg,ALT,AST,Th17 cell ratio,IL-17 and IL-23,as well as the expressions of TLR4 and p-p65 protein in liver tissues of rats in the ACY,M-CAT and H-CAT groups were significantly decreased,while the levels of serum IL-13 and IL-4 were significantly in-creased,the differences were statistically significant(P<0.05).Compared with the L-CAT and M-CAT groups,the levels of serum HB-sAg,HBeAg,ALT,AST,Th17 cell ratio,IL-17 and IL-23,the expression of liver TLR4 and p-p65 protein were significantly de-creased,while the levels of serum IL-13 and IL-4 were significantly increased in the H-CAT group,the differences were statistically significant(P<0.05).Conclusion:Catalpol improves HBV infection-induced liver injury in rats by reducing Th17 cell proportion and pro-inflammatory cytokine levels,and increasing anti-inflammatory cytokine levels,possibly by inhibiting TLR4/NF-κB p65 signaling pathway activation.关键词
乙型肝炎/梓醇/免疫反应/炎症反应Key words
Hepatitis B/Catalpol/Immune response/Inflammatory response分类
医药卫生引用本文复制引用
廖雪霞,彭鑫,黄婷婷,谢桂丹..梓醇调节TLR4/NF-κB信号通路对急性乙型肝炎大鼠肝损伤的保护作用研究[J].中国免疫学杂志,2025,41(3):545-550,6.基金项目
儋州市科技项目(QHW2020-032). （QHW2020-032）
