中国免疫学杂志2025，Vol.41Issue(3)：551-555,5.DOI:10.3969/j.issn.1000-484X.2025.03.007
酪酸梭菌干预对过敏性哮喘小鼠免疫炎症及肺部菌群的影响
Effects of Clostridium butyricum on immune inflammation and pulmonary microflora in allergic asthmatic mice
摘要
Abstract
Objective:To explore the intervention mechanism of Clostridium butyricum on asthma from the perspective of im-mune inflammation and pulmonary flora,and to provide a new scheme for the prevention and treatment of asthma.Methods:Mice were randomly divided into Clostridium butyricum(CM)group,asthma model group(Model)and normal control group(Control),with 16 mice in each group.The model group and CM group were sensitized by ovalbumin(OVA)and stimulated by continuous atomi-zation of 1%OVA solution to establish asthma mouse model,and the control group was replaced by normal saline.Mice in CM group were administrated with CM solution at 1×109 CFU/d.The total number of white blood cells in bronchoalveolar lavage fluid(BALF)was counted.Eosinophil number(EOS)was counted by Wright-Giemsa staining.The pathological changes of lung tissue were ob-served by HE staining and PAS staining.Serum levels of IL-4,IgE and IFN-γ were determined by ELISA.Mouse lung microflora was analyzed by 16S rRNA.Results:Compared with Model group,white blood cells and EOS inflammatory cells in CM group were signifi-cantly decreased(P<0.05),lung inflammatory cell infiltration and goblet cell proliferation were significantly reduced,serum IL-4 and IgE levels were significantly decreased(P<0.05),IFN-γ levels were significantly increased(P<0.05),and Th1/Th2 balance was maintained.The abundance of Proteobacteria and Escherichia-Shigella in the lung of mice were significantly decreased,and correlation analysis showed that the decrease of IL-4 and IgE levels were positively correlated with the increase of Escherichia-Shigella abundance(P<0.05,r>0.9).Conclusion:Clostridium butyricum intervention can change the composition of lung flora,regulate immune cells and cytokines,maintain Th1/Th2 balance,so as to reduce the OVA-induced pulmonary inflammation of allergic asthma.关键词
过敏性哮喘/肺部菌群/酪酸梭菌/Th1/Th2Key words
Allergic asthma/Pulmonary microflora/Clostridium butyricum/Th1/Th2分类
医药卫生引用本文复制引用
陈伟苗,王梓宁,何爽,李蓉..酪酸梭菌干预对过敏性哮喘小鼠免疫炎症及肺部菌群的影响[J].中国免疫学杂志,2025,41(3):551-555,5.基金项目
山东省医学会临床科研专项基金资助项目(YXH2022ZX01007). （YXH2022ZX01007）
