中国农业科学2025，Vol.58Issue(5)：918-928,11.DOI:10.3864/j.issn.0578-1752.2025.05.008
热激蛋白基因CfHsp70-1和CfHsp70-2在热驯化增强锈赤扁谷盗高温耐受性中的作用
The Roles of Heat Shock Protein Genes CfHsp70-1 and CfHsp70-2 in Enhancing the High-Temperature Tolerance after Heat Acclimation in Cryptolestes ferrugineus
摘要
Abstract
[Objective]Heat shock proteins(Hsps)are essential molecular chaperones in organisms and play the crucial roles in resisting adverse environmental stresses.Cryptolestes ferrugineus is a cosmopolitan pest of stored grains with strong environmental adaptability.This study aims to elucidate the crucial roles of heat shock protein genes CfHsp70-1 and CfHsp70-2 in the development of high-temperature tolerance in this pest.[Method]C.ferrugineus was acclimated to sub-lethal temperatures(37 and 42℃)for 2 h to examine the tolerance changes to lethal high temperature(50℃).Two key heat shock protein genes(CfHsp70-1 and CfHsp70-2)were identified based on the transcriptome data of C.ferrugineus,and the amino acid sequences and phylogenetic analysis of Hsp70 proteins were further conducted.The quantitative real-time PCR method was employed to analyze the expression patterns of CfHsp70-1 and CfHsp70-2 in response to sub-lethal heat stress.The RNA interference(RNAi)technology was used to silence CfHsp70-1 and CfHsp70-2,and then the changes in high-temperature tolerance of C.ferrugineus under different conditions were analyzed.[Result]The median lethal time(LT50)of different C.ferrugineus populations was significantly increased under lethal heat temperature conditions(50℃)after acclimation of insects to sub-lethal temperatures(37 and 42℃)for 2 h,indicating a substantial enhancement of the high-temperature tolerance.The further sequence and phylogenetic analysis revealed that the amino acid sequences of CfHsp70-1 and CfHsp70-2 contained three conserved Hsp70 family signature motifs,and they clustered together with Hsp70 proteins of other Coleoptera insects.The results of quantitative real-time PCR analysis suggested that the expression levels of heat shock protein genes CfHsp70-1 and CfHsp70-2 were significantly up-regulated after acclimation to 37 and 42℃for 2 h in C.ferrugineus.The gene functional analysis revealed that the high-temperature tolerance of C.ferrugineus was significantly reduced after the effective silencing of CfHsp70-1 and CfHsp70-2 via RNAi,that is,the mortality of the test insects at 50℃increased significantly.[Conclusion]The heat shock protein genes CfHsp70-1 and CfHsp70-2 are involved in enhancing the high-temperature tolerance after heat acclimation of C.ferrugineus.关键词
锈赤扁谷盗/热驯化/热激蛋白/高温耐受性/RNA干扰Key words
Cryptolestes ferrugineus/heat acclimation/heat shock protein(Hsp)/high-temperature tolerance/RNA interference(RNAi)引用本文复制引用
陈二虎,唐静杰,胡顺杰,唐培安..热激蛋白基因CfHsp70-1和CfHsp70-2在热驯化增强锈赤扁谷盗高温耐受性中的作用[J].中国农业科学,2025,58(5):918-928,11.基金项目
国家重点研发计划(2021YFD2100604)、国家自然科学基金(32272388)、江苏高校优势学科建设工程资助项目(YXK2103) （2021YFD2100604）
