中国免疫学杂志2025，Vol.41Issue(3)：556-560,5.DOI:10.3969/j.issn.1000-484X.2025.03.008
苦参碱通过调控miR-125b-5p/STAT3途径抑制人脐静脉内皮细胞增殖
Matrine inhibits proliferation of human umbilical vein endothelial cells by regulating miR-125b-5p/STAT3 pathway
摘要
Abstract
Objective:To study effect of matrine on TNF-α induced proliferation of human umbilical vein endothelial cells(HUVEC),as well as to explore whether its mechanism is related to regulation of miR-125b-5p and STAT3 expressions.Methods:HUVEC proliferation model was established by TNF-α stimulating.After matrine treatment,miR-125b-5p level was detected by real-time fluorescent quantitative PCR,proliferating cell nuclear antigen(PCNA),cell cycle protein D1(CyclinD1),matrix metallopro-teinase 2(MMP2),MMP9,STAT3 levels were detected by real-time fluorescent quantitative PCR and Western blot.Targeting rela-tionship between miR-125b-5p and STAT3 was verified by dual luciferase reporter gene assay.Results:Matrine inhibited TNF-α in-duced HUVEC proliferation(P<0.05),decreased PCNA,CyclinD1,MMP2,MMP9,STAT3 expressions(P<0.05),and increased miR-125b-5p expression(P<0.05).Overexpression of miR-125b-5p could reduce cell proliferation and PCNA,CyclinD1,MMP2,MMP9 expressions HUVEC induced by TNF-α(P<0.05).miR-125b-5p targeted STAT3 expression negatively in HUVEC,and inhi-biting miR-125b-5p expression could reverse effect of matrine on TNF-α induced cell proliferation and PCNA,CyclinD1,MMP2,MMP9 and STAT3 expressions.Conclusion:Matrine inhibits TNF-α induced proliferation of HUVEC,which is related to regulation of miR-125b-5p/STAT3 pathway.关键词
苦参碱/人脐静脉内皮细胞/增殖/miR-125b-5p/STAT3Key words
Matrine/Human umbilical vein endothelial cells/Proliferation/miR-125b-5p/STAT3分类
医药卫生引用本文复制引用
王杏,朱慧明,马欢,王超,张会欣..苦参碱通过调控miR-125b-5p/STAT3途径抑制人脐静脉内皮细胞增殖[J].中国免疫学杂志,2025,41(3):556-560,5.基金项目
河北省中医药科研计划项目(2023005) （2023005）
石家庄学院博士科研启动基金(22BS002). （22BS002）
