中国免疫学杂志2025，Vol.41Issue(3)：576-581,6.DOI:10.3969/j.issn.1000-484X.2025.03.012
lncRNA NEAT1靶向miR-495-3p对LPS诱导的WI-38细胞损伤的影响
Effects of lncRNA NEAT1 targeting miR-495-3p on WI-38 cell injury induced by LPS
摘要
Abstract
Objective:To investigate the effect of long non-coding RNA(lncRNA)nuclear enriched transcript 1(NEAT1)on lipopolysaccharide(LPS)-induced human embryonic lung cells(WI-38)injury by regulating microRNA-495-3p(miR-495-3p).Methods:WI-38 cells were treated with 10 µg/ml LPS to establish an in vitro pneumonia model.Real-time fluorescent quantitative polymerase chain reaction(qPCR)was used to detect the expression levels of NEAT1 and miR-495-3p in WI-38 cells.The dual lucif-erase reporter assay was used to detect the targeting relationship between NEAT1 and miR-495-3p.Cell counting kit and flow cytome-try were used to detect the effect of knockdown NEAT1 on the proliferation and apoptosis of WI-38 cells treated with LPS.Western blot was used to detect Bcl-2,Bax and Cleaved caspase-3 protein expressions.ELISA was used to detect the levels of inflammatory factors IL-6,IL-8 and IL-1β.Results:LPS stimulation could up-regulate the expression of NEAT1 and inhibit the expression of miR-495-3p in WI-38;NEAT1 directly targeted miR-495-3p,and NEAT1 knockdown could promote the expressions of miR-495-3p.Functionally,LPS inhibited the proliferation of WI-38 cells and promoted cell apoptosis and the expression of the inflammatory factors IL-6,IL-8 and IL-1β(P<0.05).Knockdown of NEAT1 could inhibit LPS-induced WI-38 cell apoptosis and inflammation,and promote cell pro-liferation.Conclusion:Knockdown of NEAT1 can inhibit LPS-induced apoptosis and inflammation of WI-38 cells by negatively regu-lating miR-495-3p.关键词
lncRNA NEAT1/miR-495-3p/人胚肺细胞WI-38/凋亡/炎症Key words
lncRNA NEAT1/miR-495-3p/Human embryo lung cell WI-38/Apoptosis/Inflammation分类
临床医学引用本文复制引用
朱曦,郭赟,陆涛,钱俊..lncRNA NEAT1靶向miR-495-3p对LPS诱导的WI-38细胞损伤的影响[J].中国免疫学杂志,2025,41(3):576-581,6.基金项目
江苏省医学创新团队(CXTDB2017016) （CXTDB2017016）
江苏省无锡市科技局"太湖之光"科技攻关(基础研究)(K20221033). （基础研究）
