中国免疫学杂志2025，Vol.41Issue(3)：589-594,6.DOI:10.3969/j.issn.1000-484X.2025.03.014
菌阴性肺结核中上调lncRNA MALAT1表达对巨噬细胞炎症反应的调控与机制研究
Regulation and mechanism of up-regulated lncRNA MALAT1 on macrophage inflammation in negative sputum for tuberculous bacterium
摘要
Abstract
Objective:To explore the expression and mechanism of lncRNA MALAT1 in negative sputum for tuberculous bac-terium.Methods:Expression of lncRNA MALAT1 in peripheral blood mononuclear cells(PBMC)of patients with positive sputum bacteria(Positive group)and negative sputum bacteria(Negative group)and healthy volunteers(HC group)was detected by RT-PCR.ELISA was used to detect expression levels of TNF-α,IL-1β and IL-6 in plasma.Expression of lncRNA MALAT1 in mice macro-phages RAW264.7 was silenced by siRNA interference,and RAW264.7 cells were infected with mycobacterium tuberculosis(MTB)H37Rv.Cells were divided into four groups:Control group,Control+MTB group,MTB+si-NC group and MTB+si-MALAT1 group.Proliferation activity of RAW264.7 cells in each group was detected by CCK-8 method.The number of MTB in each group was detected by CFU.Expressions of TNF-α,IL-1β and IL-6 in supernatant of RAW264.7 cells were detected by ELISA.Results:Compared with HC group,expressions of lncRNA MALAT1 in PBMC,and TNF-α,IL-1β and IL-6 in plasma were significantly increased in Positive group and Negative group(P<0.01).Compared with Control group,expression level of lncRNA MALAT1,proliferation activity,CFU value,and concentrations of TNF-α,IL-1β and IL-6 in supernatant of Control+MTB group,MTB+si-NC group and MTB+si-MALAT1 group were significantly increased(P<0.05).Compared with MTB+si-NC group,the above detection indexes in MTB+si-MALAT1 group were significantly decreased(P<0.05),while there was no significant difference in Control+MTB group(P>0.05).Conclusion:The significantly increased expression of MALAT1 in patients with negative sputum for tuberculous bacterium is positively correlated with expression of plasma inflammatory factors,while the silence of MALAT1 expression can reduce MTB induced inflammatory response by inhibiting the proliferation and phagocytosis of MTB infected macrophages.关键词
菌阴性肺结核/lncRNA MALAT1/巨噬细胞/炎症反应Key words
Negative sputum for tuberculous bacterium/lncRNA MALAT1/Macrophage/Inflammatory response分类
医药卫生引用本文复制引用
韩利梅,齐曼古力·吾守尔,刘顺苹,艾克丹木·艾尔肯,阿仙·乌日娜,关景,李新,努尔阿米娜·铁力瓦尔迪,尼格拉·伊力哈木,李晶晶..菌阴性肺结核中上调lncRNA MALAT1表达对巨噬细胞炎症反应的调控与机制研究[J].中国免疫学杂志,2025,41(3):589-594,6.基金项目
新疆维吾尔自治区自然科学基金项目(2021D01C360). （2021D01C360）
