中国免疫学杂志2025，Vol.41Issue(3)：650-654,5.DOI:10.3969/j.issn.1000-484X.2025.03.024
白藜芦醇通过NOX2介导的小胶质细胞极化减轻脑卒中后脑损伤的机制研究
Mechanism of resveratrol attenuating brain injury after ischemic stroke via NOX2-mediated polarization of microglia
摘要
Abstract
Objective:To investigate protective mechanism of resveratrol(RES)on brain injury after ischemic stroke(IS)and its regulatory mechanism on nico-tinamide adenine dinucleotide phosphate oxidase 2(NOX2)mediated polarization of microglia.Methods:Low and high doses of RES were used for intervention.Tetrabromocinnamic acid(TBCA),a specific agonist of NOX2,was used to save function.PC12 and LMAI Bio cell models with oxygen-glucose deprivation/reoxygenation(OGD/R)were constructed.TUNEL was used to detect apoptosis of PC12 cells.Immunofluorescence was used to detect polarization of LMAI Bio cells.Western blot was used to detect expressions of NOX2,NF-κB,TNF-α,IL-1β,IL-10 and TGF-β in PC12 and LMAI Bio cells.Results:RES could significantly inhibit apoptosis of PC12 cells induced by OGD/R,and regulate LMAI Bio cells polarization to M2 type.Western blot showed that RES could significantly down-regulate NOX2,NF-κB,TNF-α and IL-1β expressions in OGD/R cells,up-regulate IL-10 and TGF-β expressions,while TBCA could partially reverse protective effect of RES on neurons.Conclusion:RES can inhibit neuronal apoptosis after IS,which may be related to its regulation of NOX2-mediated polarization of microglia.关键词
白藜芦醇/脑卒中/烟酰胺腺嘌呤二核苷酸磷酸氧化酶2/小胶质细胞极化Key words
Resveratrol/Ischemic stroke/Nico-tinamide adenine dinucleotide phosphate oxidase 2/Microglial polarization分类
医药卫生引用本文复制引用
何小群,罗春花,黄灵,肖刚..白藜芦醇通过NOX2介导的小胶质细胞极化减轻脑卒中后脑损伤的机制研究[J].中国免疫学杂志,2025,41(3):650-654,5.基金项目
广西壮族自治区卫生健康委员会科研课题(Z20200023) （Z20200023）
广西中医药管理局科研课题(GZZC2020249). （GZZC2020249）
