中国免疫学杂志2025，Vol.41Issue(3)：655-660,6.DOI:10.3969/j.issn.1000-484X.2025.03.025
2型固有淋巴细胞(ILC2)在布鲁氏菌感染中的变化特点研究
Study on changes of type 2 intrinsic lymphocyte(ILC2)in Brucella infection
摘要
Abstract
Objective:To investigate variation and correlation of type 2 intrinsic lymphocyte(ILC2)and related factors in acute and chronic brucellosis,to identify immune role of ILC2 in chronic brucellosis.Methods:Forty-three patients with acute brucel-losis and 45 patients with chronic brucellosis were compared with 49 healthy controls.ILC2 level in each group was detected by flow cytometry.mRNA level of GATA3 in PBMC was detected by fluorescence quantitative PCR.Serum IL-33,ST2,IL-4 and IL-13 levels were detected by ELISA.ALT and AST levels were measured by fully automatic biochemical analyzer.Results:ILC2 level,GATA3 mRNA in PBMC,serum IL-33,IL-4 and IL-13 levels in chronic brucellosis group were significantly higher than healthy control group and acute brucellosis group(P<0.01).Correlation analysis showed that ILC2 level was positively correlated with GATA3 mRNA,IL-33,IL-4 and IL-13 levels,while negatively correlated with ST2 level.ROC curve suggested that ILC2,GATA3,IL-33 and ST2 had good predictive ability for severity of brucellosis patients(AUC>0.7).Conclusion:Increase of ILC2 and its related factors are closely related to chronic brucella infection,which may play an important immune role in pathogenesis of brucella infection.关键词
布鲁氏菌病/2型固有淋巴细胞/GATA3/IL-33/IL-4/IL-13Key words
Brucella infection/Type 2 intrinsic lymphocyte/GATA3/IL-33/IL-4/IL-13分类
医药卫生引用本文复制引用
殷郑伟,朱玥洁,史娟,何月月,丁剑冰,王泉,张峰波..2型固有淋巴细胞(ILC2)在布鲁氏菌感染中的变化特点研究[J].中国免疫学杂志,2025,41(3):655-660,6.基金项目
国家自然科学基金(82360394) （82360394）
新疆维吾尔自治区杰出青年项目(2023D01E12) （2023D01E12）
新疆维吾尔自治区科技援疆计划(2022E02061) （2022E02061）
新疆维吾尔自治区青年科技拔尖人才计划(2022TSYCCX0112). （2022TSYCCX0112）
