中国实用口腔科杂志2025，Vol.18Issue(2)：160-169,10.DOI:10.19538/j.kq.2025.02.006
三种普雷沃菌致龋效果比较研究
Comparative study on the cariogenic effects of three species of Prevotella
摘要
Abstract
Objective To investigate and compare the cariogenic effects of three Prevotella species[Prevotella histicola(P.h),Prevotella denticola(P.d)and Prevotella maculosa(P.m)]and provide a reference basis for the pre-vention and treatment of dental caries.Methods Three Prevotella species and Streptococcus mutans(S.m)were cultured for 24 h.The pH of the bacterial supernatant was then measured,and high-performance liquid chromatography was employed to detect acid production in the supernatant.The four bacterial cultures were incubated at pH levels of 4.5,5.5,and 6.5 for 24 h,and the optical density(OD)was mea-sured to evaluate bacterial acid resistance.Crystal violet staining was used to detect the biofilm formation of the four bac-terial cultures after 48 h of incubation.The morphology and structure of the biofilm were observed using scanning elec-tron microscopy and laser confocal microscopy.The content of water-insoluble extracellular polysaccharides in the bio-film was determined using the anthrone method.Twenty impacted third molars were collected from patients who visited the Department of Stomatology at Qingdao Municipal Hospital between July and August 2024.Forty enamel blocks were prepared and randomly divided into five groups,each containing eight blocks.The groups were incubated in TSBS cul-ture medium with bacterial solutions of P.h(P.h group),P.d(P.d group),P.m(P.m group),and S.m(S.m group),and a sterile control group was set.After two weeks of culture,the enamel surface morphology was examined using scanning electron microscope,and the difference in Vickers hardness(HV)of the enamel blocks before and after culturing was measured with a micro Vickers hardness tester.Results ① After 24 h of incubation,compared with the three Prevotella species,the pH of the supernatant from S.m was lower,and the concentrations of lactic acid and total acid were higher.The pH of the supernatant from P.h was significantly lower than that from P.d and P.m,and the concentrations of succin-ic acid,lactic acid,acetic acid,and total acid were all greater than those of P.d and P.m,with all differences being statis-tically significant(P＜0.05).The differences between P.d and P.m were not statistically significant(P＞0.05).②After being cultured for 24 h under different pH conditions,the OD of S.m significantly increased compared to the three Pre-votella species.At a pH of 6.5,the OD of P.h was greater than that of both P.d and P.m,while the OD of P.d exceeded that of P.m,with all differences being statistically significant(P＜0.05).As the values of pH decreased,bacterial growth was progressively inhibited.At a pH of 4.5,the OD of S.m increased slowly,while the growth of the three Prevotella spe-cies was greatly inhibited.③After 48 h of cultivation,all three species of Prevotella were capable of forming biofilms.The biofilm formed by P.h exhibited a uniform and dense structure,contaning numerous microbial colonies.Compared to those of P.d and P.m,the concentration of insoluble extracellular polysaccharides in the P.h biofilm[(1.39±0.23)g/L]was significantly higher,along with an increased biofilm thickness[(16.33±2.08)μm],all the differences being statisti-cally significant(P＜0.05).The biofilm thicknesses for P.d and P.m were(8.33±1.53)μm and(7.33±1.53)μm,re-spectively,with corresponding concentrations of water-insoluble extracellular polysaccharides being(0.91±0.04)g/L and(0.72±0.08)g/L.No significant differences were observed between these two species(both P＞0.05).④A11 three species of Prevotella were capable of inducing enamel demineralization.Specifically,the P.h group exhibited a more se-vere degree of enamel surface demineralization,characterized by a larger HV difference than that of P.d and P.m,the dif-ferences being statistically significant(P＜0.05).There was no statistically significant difference in HV difference be-tween the P.d group and the P.m group(P＞0.05).Moreover,the HV difference in the S.m group exceeded three Prevotel-la groups,with statistically significant differences(all P＜0.05).Conclusion P.h,P.d and P.m can all lead to dental caries through acid production,acid tolerance,biofilm formation and induction of enamel demineralization,although their cariogenicity is slightly weaker than S.m.The cariogenic effect of P.h is greater than the other two Prevotella species,while no significant differences in cariogenicity are observed between P.d and P.m.关键词
栖组织普雷沃菌/栖牙普雷沃菌/小斑点普雷沃菌/牙釉质龋/生物膜/脱矿Key words
Prevotella histicola/Prevotella denticola/Prevotella maculosa/enamel caries/biofilm/demineralization分类
口腔医学引用本文复制引用
赵晓芳,陶匀雅,孙雁斐,孙翔琨,梅昆洲,张雅萌,崔严文,杨芳..三种普雷沃菌致龋效果比较研究[J].中国实用口腔科杂志,2025,18(2):160-169,10.基金项目
国家自然科学基金(81670979) （81670979）
