中国中医药科技2025，Vol.32Issue(2)：211-215,5.
苍耳子含药血清对IgE致肥大细胞脱颗粒、炎性因子释放及PLCγ、PKC表达的影响
Effects of Cang'erzi(Xanthium xanthii)-Containing Serum on Mast Cells Degranulation Induced by IgE and Infalmmatory Factors,PLCγ、PKC Expressions
摘要
Abstract
Objective:To investigate the effects of Xanthium xanthii-containing serum on degranulation of Mast Cells(MCs)byregulating PLCγ/PKCexpressions.Methods:RBL-2H3 cellswereroutinelyculturedand sensitized with IgE,experiment was divided into control group,model group,Xanthium xanthii-containing serum group and Ketotifen fumarate group(positive group)to receive corresponding drug intervention.Determination of calcium influx in the supernatant of RBL-2H3 cell culture medium by immunofluorescence staining.ELISA to detect changes in the levels of the inflammatory factors TNF-α,IL-6 release and β-aminohexosidase(β-Hex).Western blot was used to detect the expressions of phosphorylated T-cell-activated connexin(p-LAT),phosphorylated phosphodiesterase Cα1(p-PLCα1),and p-PKC proteins.Results:Compared with the control group,calcium influx,contents of TNF-α and IL-6,cell degranulation ability,fluorescence expression intensity of PKCα and protein expression levels of p-LAT,p-PLCα1 and p-PKC in model group were significantly increased(P<0.01).Compared with model group,calcium influx,TNF-α and IL-6 contents,cell degranulation ability and fluorescence expression intensity of PKCα in Xanthium xanthii-containing serum group and Ketotifen fumarate group were significantly decreased(P<0.01).The protein expression levels of p-LAT,p-PLCα1 and p-PKC were significantly decreased(P<0.05 or P<0.01).Conclusion:The Xanthium xanthii-containing serum can regulate MCs degranulation through PLCγ/PKC/Ca2+signaling pathway.关键词
苍耳子含药血清/肥大细胞脱颗粒/PLCγ/PKC/Ca2+信号通路/TNF-α/IL-6/体外实验Key words
Xanthium xanthii-containing serum/mast cell degranulation/PLCγ/PKC/Ca2+signaling pathway/TNF-α/IL-6/in vitro experiment引用本文复制引用
周淑贞,邵深深..苍耳子含药血清对IgE致肥大细胞脱颗粒、炎性因子释放及PLCγ、PKC表达的影响[J].中国中医药科技,2025,32(2):211-215,5.基金项目
浙江省温州市基础医疗卫生科技项目(Y20180817) （Y20180817）
