中国中医药科技2025，Vol.32Issue(2)：216-221,6.
畲药地稔抗大鼠Hp相关慢性非萎缩性胃炎的实验研究
Experimental Study on Antagonism Effect of She Medicine Diren(Melastoma dodecandrum)on Hp-Related Chronic Non-Atrophic Gastritis in Rats
摘要
Abstract
Objective:To investigate the antagonism effect of Melastoma dodecandrum on Hp-related chronic non-atrophic gastritis.Methods:60 SD rats were divided into normal control group,model group,positive group,low,medium and high dose of Melastoma dodecandrum groups with 10 rats in each group.Except thecontrol group,the rest rats were modeled by amoxicillin+Hp gavage.Rats in normal group and model group were intragastrically given saline every day,the positive group was intragastrically given Amoxicillin at 100 mg/kg,and the Melastoma dodecandrum groups were intragastrically given 400,2 000,4 000 mg/kg Melastoma dodecandrum water decoction,respectively,for 14 days.The contents of IL-1β,IL-2,TNF-α,IFN-β,CRP in serum of rats were detected by ELISA,the pathological changes of gastric mucosa were detected by transmission electron microscopy,and the expression levels of inflammatory factors and Hp gene in gastric mucosa were detected by real-time fluorescence quantitative PCR.Western Blotting was used to detect inflammatory factor proteins in gastric mucosa of rats.Results:The contents of IL-1β,IL-2,TNF-α,IFN-β,CRP in serum of rats could be significantly decreased in medium and high dose of Melastoma dodecandrum groups,while the mRNA and protein expression levels of IL-1β,IL-2,TNF-α,IFN-β,CRP and urease in gastric mucosa of rats were significantly decreased in high dose of Melastoma dodecandrum group.The pathological changes of gastric mucosa in low,medium and high doses of Melastoma dodecandrum were improved.Conclusion:Melastoma dodecandrum can effectively treat HP-related chronic non-atrophic gastritis in rats,and its mechanism may be related to inhibiting inflammatory factors.关键词
地稔/Hp相关慢性非萎缩性胃炎/病理学/尿素酶/炎症因子/大鼠Key words
Melastoma dodecandrum/Hp-related chronic non-atrophic gastritis/pathology/urease/inflammatory factors/rat引用本文复制引用
周继旺,雷后兴,李丽乐,卢仙宝,吴建业,刘爽,张晓芹..畲药地稔抗大鼠Hp相关慢性非萎缩性胃炎的实验研究[J].中国中医药科技,2025,32(2):216-221,6.基金项目
浙江省丽水市公益性技术应用研究项目(2022GYX69) （2022GYX69）
浙江省丽水市重点学科"畲医药学" （）
