中国医院用药评价与分析2025，Vol.25Issue(3)：266-273,278,9.DOI:10.14009/j.issn.1672-2124.2025.03.003
基于生物网络探究三七治疗类风湿关节炎的作用机制
Mechanism of Panax notoginseng in the Treatment of Rheumatoid Arthritis Based on Biological Network
摘要
Abstract
OBJECTIVE:To analyze the core genes of rheumatoid arthritis based on bioinformatics,and explore the mechanism of anti-rheumatoid arthritis effects of Panax notoginseng through network pharmacology and molecular docking.METHODS:The active components of Panax notoginseng were searched in Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform,Traditional Chinese Medicine Integrated Database,and HERB based on the bioavailability,and the corresponding targets were predicted by Swiss Target Prediction.The rheumatoid arthritis gene chips obtained from GEO database were analyzed,and the differentially expressed genes(DEGs)associated with rheumatoid arthritis were obtained by bioinformatics method,and the core genes associated with rheumatoid arthritis were analyzed.The drug-component-target network was constructed by taking the common targets of Panax notoginseng and rheumatoid arthritis,and the enrichment analysis of the intersection genes was performed.The binding affinity between the core components of Panax notoginseng and core targets of rheumatoid arthritis was verified by molecular docking.RESULTS:The bioinformatics analysis showed that the hub genes of rheumatoid arthritis included Vav Guanine Nucleotide Exchange Factor 1,CD8α Molecule(CD8A),and Spleen Tyrosine Kinase(SYK).Results of network pharmacology showed that 58 major chemical components of Panax notoginseng could act on 53 targets of rheumatoid arthritis,and the core targets included LCK,CXCR4,EGFR,GZMB,PPARG,CD8A,MMP9,SYK,ITGAL,CXCR3,and FASN.The enrichment analysis of 53 intersection genes showed that the major active components of Panax notoginseng may participate in multiple signaling pathways through various biological mechanisms to exert anti-rheumatoid arthritis effects.Molecular docking also indicated that the core active components of Panax notoginseng had good binding ability to the hub genes of rheumatoid arthritis.CONCLUSIONS:Panax notoginseng can exert anti-rheumatoid arthritis effects through multiple components,multiple targets,and multiple pathways.关键词
三七/类风湿关节炎/生物信息学Key words
Panax notoginseng/Rheumatoid arthritis/Bioinformatics分类
药学引用本文复制引用
邓茜,彭江云,王宏,李湘媛,彭紫凝,毛丹宁,孟凡雨,黄元波,晏蔚田,刘念,邓春玲..基于生物网络探究三七治疗类风湿关节炎的作用机制[J].中国医院用药评价与分析,2025,25(3):266-273,278,9.基金项目
国家自然科学基金资助项目(No.82160901) （No.82160901）
云南省教育厅科学研究基金项目(No.2023Y0463) （No.2023Y0463）
