中国医院用药评价与分析2025，Vol.25Issue(3)：282-288,7.DOI:10.14009/j.issn.1672-2124.2025.03.006
川芎提取物改善曲妥珠单抗诱导心肌细胞损伤的药效物质研究
Pharmacoactive Substances of Chuanxiong Rhizoma Extract in Ameliorating Trastuzumab-Induced Myocardial Cell Injury
摘要
Abstract
OBJECTIVE:To explore the underlying mechanism of Chuanxiong rhizoma extract in ameliorating trastuzumab-induced cardiotoxicity.METHODS:Homogeneous design and in vitro bioactivity assay for protection against myocardial cell injury were applied,combined with chemical fingerprinting for spectral correlation analysis,the pharmacodynamic components of Chuanxiong rhizoma extract in ameliorating trastuzumab-induced cardiomyocyte injury were explored.RESULTS:Homogeneous design showed that the optimal ethanol extraction process of Chuanxiong rhizoma was 75%ethanol,9 times of solvent volume,ethanol infiltration for 35 minutes and ultrasonic extraction for 30 minutes.Under homogeneous design test,extracts of samples 1 to 9 could ameliorate trastuzumab-induced myocardial cell injury,the strongest protective effect was S3(EC50=0.037),and the weakest was S2(EC50=0.317).Results of correlation analysis showed that tetramethylpyrazine,tetramethylpyrolactone I,ferulic acid and n-butylphthalide were correlated,and tetramethylpyrazine had the strongest correlation(r=0.68,P<0.05).Validation experiments showed that ligustrazine had the strongest activity,followed by ferulic acid,n-butylphthalide and ligustractone I.The results were in agreement with the spectral correlation analysis.CONCLUSIONS:The alcohol exvtraction method can affect the content of Chuanxiong rhizoma extract in ameliorating trastuzumab-induced myocardial cell injury.Ligustrazine,ligustractone I,ferulic acid and n-butylphthalide are the main active components of Chuanxiong rhizoma extract in improving trastuzumab-induced myocardial cell injury.This study may provide a scientific basis for the quality control method of traditional Chinese medicine to improve cardiotoxicity associated with anticancer drugs.关键词
乳腺癌/曲妥珠单抗/心肌细胞损伤/川芎提取物/谱效相关分析Key words
Breast cancer/Trastuzumab/Myocardial cell injury/Chuanxiong Rhizoma extract/Spectral effect correlation分类
医药卫生引用本文复制引用
杨家慧,徐静,刘欣桐,曹波,余诺贤,张铭予,范琳琳,李春雨,李国辉..川芎提取物改善曲妥珠单抗诱导心肌细胞损伤的药效物质研究[J].中国医院用药评价与分析,2025,25(3):282-288,7.基金项目
北京市自然科学基金资助项目(No.L248025) （No.L248025）
