植物学报2025，Vol.60Issue(2)：235-245,11.DOI:10.11983/CBB24061
野蔷薇组培快繁和高效瞬时表达体系的建立
Tissue Culture,Rapid Propagation and Efficient Transient Expression Systems of Rosa multiflora
摘要
Abstract
A rapid propagation system via tissue culture for Rosa multiflora was established using the stem segments with buds of the current-year as the experimental material.The results showed that the best explants were stem segments with axillary buds.The best disinfection method was to soak the explants in 75％ethanol for 30 seconds,and then soak them in 10％sodium hypochlorite solution for 20 minutes.The survival rate can reach 96％.The optimal bud-induction medium was MS+1.0 mg·L-1 6-BA+0.01 mg·L-1 NAA+0.1 mg·L-1 GA3.The budding rate can reach 98％after 30 days of cultivation.WPM was the best basal medium for the proliferation of sterile regenerated plantlets,and the proliferation coefficient was 2.87.The best medium for rooting was 1/2MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 NAA,and the rooting rate can reach 93％.The transplanting survival rate of sterile regenerated plantlets was 98％.On this basis,the transient expres-sion system of R.multiflora was established.The results showed that the optimal transformation conditions for transient expression were OD600 of 0.8 for the bacterium culture medium,vacuum negative pressure of-0.10 MPa and vacuum suction twice for 15 minutes each time.The transient expression efficiency can reach 96％.The results of this study laid a foundation for the establishment of regeneration and genetic transformation system of R.multiflora,and also provided technical support for studying on the gene function of Rosa plants.关键词
野蔷薇/快繁/无菌再生苗/真空渗透/瞬时表达Key words
Rosa multiflora/rapid propagation/sterile regenerated plantlets/vacuum infiltration/transient expression引用本文复制引用
曹雪敏,包颖,张悦新,李瑞杰,苏健馨,张蔚..野蔷薇组培快繁和高效瞬时表达体系的建立[J].植物学报,2025,60(2):235-245,11.基金项目
国家自然科学基金(No.32072617)、河北省自然科学基金(No.C2021105002)和河北省现代农业产业技术体系建设专项(No.HBCT2024200405) （No.32072617）
