植物学报2025，Vol.60Issue(2)：246-255,10.DOI:10.11983/CBB24143
马来甜龙竹多倍体高效诱导及鉴定
Efficient Induction and Identification of Polyploids in Dendrocalamus asper
摘要
Abstract
Due to the long flowering cycle,unpredictable flowering period and low seed setting rate of most bamboo plants,bamboo breeding has been a challenge in the research of bamboo plants.Polyploid breeding,as a common mean of plant breeding,is able to obtain progeny with excellent traits through artificial induction.In bamboo breeding,there are fewer studies on polyploid breeding.In this study,based on the existing regeneration system of Dendrocalamus asper,the embryonic calluses of D.asper were treated with colchicine using the liquid suspension method and the solid medium mixed culture method,respectively.The results showed that,based on the differentiation and browning rates of the cal-luses,the better results were obtained by treating the calluses with 50 mg·L-1 colchicine for 48-72 hours using the liquid suspension method.A total of 54 regenerated plants,including 7 control plants,and 16 chromosome doubled plants were successfully obtained from D.asper using flow cytometry to test all the regenerated plants.In terms of chromosomal doubling,treatment with 100 mg·L-1 colchicine for 48 h produced the highest number of chromosome-doubled plants with a polyploidy rate of 54.54％.Compared with 6-ploid plants,the 12-ploid plants presented larger and thicker leaves,and larger lower epidermal stomata,implying their superiority in stress tolerance physiology.This study provides an efficient polyploid breeding technique based on the in vitro indirect regeneration system of bamboo,and offers a new solution for breeding new polyploid germplasm of bamboo.关键词
马来甜龙竹/秋水仙素/愈伤组织/倍性分析/多倍体鉴定Key words
Dendrocalamus asper/colchicine/callus/ploidy analysis/identification of polyploid引用本文复制引用
郭政,邵香君,鲁海雯,侯丹,孔思梦,李翔宇,刘华倩,林新春..马来甜龙竹多倍体高效诱导及鉴定[J].植物学报,2025,60(2):246-255,10.基金项目
国家重点研发计划(No.2021YFD2200503-3)和大学生创新创业教育基金(No.202210341015) （No.2021YFD2200503-3）
