植物学报2025，Vol.60Issue(2)：283-293,11.DOI:10.11983/CBB24056
基于FRET原理的生物传感器:小分子荧光探针在植物中的研究进展
FRET-based Biosensors:Application of Small Molecule Fluorescence Probes in Plants
摘要
Abstract
Biological small molecules,also known as monomeric compounds with relatively low molecular weight found in organisms,encompass a wide array of substances in plants,such as ions,plant hormones and metabolites.Studying the dynamic fluctuations of these small molecules in plants is crucial for analyzing their corresponding physiological func-tions,regulatory networks,and enhancing the precision of botanical research.Genetically encoded fluorescent biosen-sors/probes utilizing Förster resonance energy transfer(FRET)technology serves as a valuable tool for real-time moni-toring of these small molecules within living organisms.These FRET biosensors/probes allow for the non-invasive visu-alization of specific small molecule concentrations,providing detailed information at a high resolution.Because of these unique advantages,this technique has been extensively applied in various research fields,including plant physiology,developmental biology,and environmental science.This review provides a comprehensive overview of FRET sen-sors/probes utilized in plant research in recent years,outlines the key design concepts,and highlights their applications and advances in detecting ions,plant hormones,and metabolites.Furthermore,this review demonstrates practical tech-nological tools and potential research directions for elucidating the functions of small biomolecules in plants.关键词
生物传感器/FRET/生物小分子/植物细胞Key words
biosensors/FRET/biological small molecules/plant cell引用本文复制引用
吕加一,李乐攻,侯聪聪..基于FRET原理的生物传感器:小分子荧光探针在植物中的研究进展[J].植物学报,2025,60(2):283-293,11.基金项目
国家自然科学基金重点项目(No.31930010) （No.31930010）
