中国药业2025，Vol.34Issue(7)：45-50,6.DOI:10.3969/j.issn.1006-4931.2025.07.010
司美格鲁肽改善糖尿病肾病模型小鼠肾功能损伤的作用机制
Mechanism of Semaglutide in Improving Renal Function Damage in Diabetes Nephropathy Model Mice
摘要
Abstract
Objective To investigate the mechanism of smeglutide in improving renal function damage in diabetes nephropathy(DN)model mice.Methods A total of 30 SPF mice were randomly selected and fed with high-fat diet(66.5%maintenance feed+10%lard+2.5%cholesterol+1%sodium cholate)for six weeks,and then they were intraperitoneally injected with 50 mg/kg streptozotocin solution to replicate the mice model.Another 10 mice were selected and fed with the regular diet.The mice fed with regular diet were set as the blank control group(group A,equal amount of normal saline),and the successfully modeled mice were randomly divided into the model control group(group B,equal amount of normal saline),the semaglutide group(group C,60 µg/kg)and liraglutide group(group D,200 µg/kg),with 10 mice in each group,and they were intraperitoneally injected with the corresponding drugs for eight weeks.The levels of fasting blood glucose(FBG),serum creatinine(SCr),urea nitrogen(BUN),and the 24 h urinary protein quantification(24 h UTP)in mice were measured.The pathological change of renal tissue was observed by the hematoxylin-eosin(HE)staining method.The expression levels of serum interleukin(IL)-18 and IL-1β were detected by the enzyme-linked immunosorbent assay(ELISA).The expression of nuclear factor-κB p65(NF-κB p65),NOd-like receptor protein 3(NLRP3)and caspase-1 in renal tissues were detected by the Western blot.Results Compared with those in group B,the levels of FBG,serum SCr,and BUN,the 24 h UTP,renal pathology score,the expression levels of IL-18 and IL-1β,and the expression levels of NF-κB p65,NLRP3,and caspase-1 protein in group C were significantly lower(P<0.05);the renal pathological injury in group C significantly improved(P<0.05).Conclusion Simeglutide can significantly improve renal function injury in DN model mice,and its mechanism may be related to regulating the NF-κB/NLRP3 signaling pathway,reducing inflammatory response,and inhibiting cell apoptosis.关键词
司美格鲁肽/糖尿病肾病/胰高血糖素样肽-1受体激动剂/核因子-κB/核苷酸结合结构域富含亮氨酸重复序列和含热蛋白结构域受体3信号通路Key words
semaglutide/diabetes nephropathy/glucagon like peptide-1 receptor agonist/NF-κB/NLRP3 signaling pathway分类
医药卫生引用本文复制引用
姜啸,姜玫,王智峰,赵涛,曹思明,李秋梅..司美格鲁肽改善糖尿病肾病模型小鼠肾功能损伤的作用机制[J].中国药业,2025,34(7):45-50,6.基金项目
辽宁省大连市医学科学研究计划项目[2211041]. （）
